Association of Bcl-2 with cyclin A/Cdk-2 complex and its effects on Cdk-2 activity

In the human endometrial carcinoma cell line HEC1B, the overexpression of Bc1-2 leads to a G(2)/M cell cycle arrest. The experiments presented in this work suggest a direct interaction between the antiapoptotic protein Bcl-2 and the cyclin-dependent kinase Cdk-2 and suggest that such interaction is cell cycle dependent. While further experiments are currently under way in our laboratory to elucidate the significance of Cdk-2/Bcl-2 complexes in PCD and cell cycle regulation, we demonstrate also a specific inhibitory function of Bcl-2 on the activity of the coprecipitated kinase.