Analysis of a cAMP-responsive activator reveals a two-component mechanism for transcriptional induction via signal-dependent factors

被引：211

作者：

Nakajima, T

Uchida, C

Anderson, SF

Parvin, JD

Montminy, M

机构：

[1] HARVARD UNIV,SCH MED,DEPT CELL BIOL,BOSTON,MA 02115

[2] HARVARD UNIV,SCH MED,BRIGHAM & WOMENS HOSP,DEPT PATHOL,BOSTON,MA 02115

来源：

GENES & DEVELOPMENT | 1997年 / 11卷 / 06期

关键词：

cAMP responsive activator; CREB; transcriptional activation RNA Pol II; signal-dependent factors;

D O I：

10.1101/gad.11.6.738

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have examined the mechanism by which the cAMP-responsive factor CREB stimulates target gene expression following its phosphorylation at Ser-133. Using an in vitro transcription assay, we found that two signals were required for target gene activation: a phospho(Ser-133)-dependent interaction of CREB with RNA polymerase II via the coactivator CBP and a glutamine-rich domain interaction with TFIID via hTAF(II)130. The adenovirus E1A oncoprotein was found to inhibit phospho(Ser-133) CREB activity by binding to CBP and specifically blocking recruitment of RNA Pol II to the promoter. Our results suggest that the recruitment of CBP-RNA Pol II complexes per se is not sufficient for transcriptional activation and that activator-mediated recruitment of TFIID is additionally required for induction of signal-dependent genes.

引用

页码：738 / 747

页数：10

共 30 条

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