Probing the structural dynamics of nucleic acids by quantitative time-resolved and equilibrium hydroxyl radical 'footprinting'

被引：76

作者：

Brenowitz, M

Chance, MR

Dhavan, G

Takamoto, K

机构：

[1] Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10461 USA

[2] Albert Einstein Coll Med, Dept Physiol & Biophys, Ctr Synchrotron Biosci, Bronx, NY 10461 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2002年 / 12卷 / 05期

关键词：

D O I：

10.1016/S0959-440X(02)00366-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

For many years, hydroxyl radical footprinting has been an insightful probe of the solvent accessibility of local regions of nucleic acid structure. Recently, quantitative applications of this technique have been developed that allow time-resolved and equilibrium analysis of transitions involving nucleic acid ligand binding and conformation change to be analyzed incisively.

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页码：648 / 653

页数：6

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