Quantitative Determination of the Conformational Properties of Partially Folded and Intrinsically Disordered Proteins Using NMR Dipolar Couplings

被引：130

作者：

Jensen, Malene Ringkjobing ^{[1
]}

Markwick, Phineus R. L. ^{[1
]}

Meier, Sebastian ^{[2
]}

Griesinger, Christian ^{[3
]}

Zweckstetter, Markus ^{[3
]}

Grzesiek, Stephan ^{[2
]}

Bernado, Pau ^{[1
]}

Blackledge, Martin ^{[1
]}

机构：

[1] CEA, CNRS, UJF, Inst Biol Struct,UMR 5075, F-38027 Grenoble, France

[2] Max Planck Inst Biophys Chem, Dept NMR Based Struct Biol, D-37077 Gottingen, Germany

[3] Univ Basel, Biozentrum, CH-4056 Basel, Switzerland

来源：

STRUCTURE | 2009年 / 17卷 / 09期

关键词：

NATIVELY UNFOLDED PROTEINS; 8 M UREA; LONG-RANGE INTERACTIONS; CHEMICAL-SHIFT DATA; X-RAY-SCATTERING; ALPHA-SYNUCLEIN; SECONDARY STRUCTURE; DENATURED STATE; UNSTRUCTURED PROTEINS; MOLECULAR RECOGNITION;

D O I：

10.1016/j.str.2009.08.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intrinsically disordered proteins (IDPs) inhabit a conformational landscape that is too complex to be described by classical structural biology, posing an entirely new set of questions concerning the molecular understanding of functional biology. The characterization of the conformational properties of IDPs, and the elucidation of the role they play in molecular function, is therefore one of the major challenges remaining for modern structural biology. NMR is the technique of choice for studying this class of proteins, providing information about structure, flexibility, and interactions at atomic resolution even in completely disordered states. In particular, residual dipolar couplings (RDCs) have been shown to be uniquely sensitive and powerful tools for characterizing local and long-range structural behavior in disordered proteins. In this review we describe recent applications of RDCs to quantitatively describe the level of local structure and transient long-range order in IDPs involved in viral replication, neurodegenerative disease, and cancer. © 2009 Elsevier Ltd. All rights reserved.

引用

页码：1169 / 1185

页数：17

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