Gene sensor using ferrocenyl oligonucleotide

被引：197

作者：

Ihara, T ^{[1
]}

Nakayama, M ^{[1
]}

Murata, M ^{[1
]}

Nakano, K ^{[1
]}

Maeda, M ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC ENGN,DEPT CHEM SCI & TECHNOL,HIGASHI KU,FUKUOKA 81281,JAPAN

来源：

CHEMICAL COMMUNICATIONS | 1997年 / 17期

关键词：

D O I：

10.1039/a703401f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An electrochemical gene sensing system has been developed using a redox-active ferrocene-modified oligonucleotide and an oligonucleotide anchored on a gold electrode, both of which formed a sandwich-type ternary complex with the target DNA to give a redox current.

引用

页码：1609 / 1610

页数：2

共 15 条

[1] Trace measurements of plasmid DNAs by adsorptive stripping potentiometry at carbon paste electrodes [J].

Cai, XH ;

Rivas, G ;

Farias, PAM ;

Shiraishi, H ;

Wang, J ;

Fojta, M ;

Palecek, E .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1996, 40 (01) :41-47

[2] SEQUENCE-SPECIFIC GENE DETECTION WITH A GOLD ELECTRODE MODIFIED WITH DNA PROBES AND AN ELECTROCHEMICALLY ACTIVE DYE [J].

HASHIMOTO, K ;

ITO, K ;

ISHIMORI, Y .

ANALYTICAL CHEMISTRY, 1994, 66 (21) :3830-3833

[3] Ferrocene-oligonucleotide conjugates for electrochemical probing of DNA [J].

Ihara, T ;

Maruo, Y ;

Takenaka, S ;

Takagi, M .

NUCLEIC ACIDS RESEARCH, 1996, 24 (21) :4273-4280

[4] 3H-1,2-BENZODITHIOLE-3-ONE 1,1-DIOXIDE AS AN IMPROVED SULFURIZING REAGENT IN THE SOLID-PHASE SYNTHESIS OF OLIGODEOXYRIBONUCLEOSIDE PHOSPHOROTHIOATES [J].

IYER, RP ;

EGAN, W ;

REGAN, JB ;

BEAUCAGE, SL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (03) :1253-1254

[5] Comparison of an intercalating dye and an intercalant-enzyme conjugate for DNA detection in a microtiter-based assay [J].

Kolakowski, B ;

Battaglini, F ;

Lee, YS ;

Klironomos, G ;

Mikkelsen, SR .

ANALYTICAL CHEMISTRY, 1996, 68 (07) :1197-1200

[6] MUTATION DETECTION BY MISMATCH BINDING-PROTEIN, MUTS, IN AMPLIFIED DNA - APPLICATION TO THE CYSTIC-FIBROSIS GENE [J].

LISHANSKI, A ;

OSTRANDER, EA ;

RINE, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (07) :2674-2678

[7] DNA-IMMOBILIZED GOLD ELECTRODE FOR DNA-BINDING DRUG SENSOR [J].

MAEDA, M ;

MITSUHASHI, Y ;

NAKANO, K ;

TAKAGI, M .

ANALYTICAL SCIENCES, 1992, 8 (01) :83-84

[8]

Mikkelsen Susan R., 1996, Electroanalysis, V8, P15, DOI 10.1002/elan.1140080104

[9] Synthesis and characterization of DNA with ferrocenyl groups attached to their 5'-termini: Electrochemical characterization of a redox-active nucleotide monolayer [J].

Mucic, RC ;

Herrlein, MK ;

Mirkin, CA ;

Letsinger, RL .

CHEMICAL COMMUNICATIONS, 1996, (04) :555-557

[10]

Palanti S, 1996, ANAL LETT, V29, P2309

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