A novel marine compound xyloketal B protects against oxidized LDL-induced cell injury in vitro

被引：79

作者：

Chen, Wen-Liang ^{[1
]}

Qian, Yan ^{[1
]}

Meng, Wei-Feng ^{[1
]}

Pang, Ji-Yan ^{[2
,3
]}

Lin, Yong-Cheng ^{[2
,3
]}

Guan, Yong-Yuan ^{[1
]}

Chen, Sheng-Pin ^{[3
]}

Liu, Jie ^{[1
]}

Pei, Zhong ^{[4
]}

Wang, Guan-Lei ^{[1
,3
]}

机构：

[1] Sun Yat Sen Univ, Dept Pharmacol, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Dept Appl Chem, Guangzhou 510080, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, Dept Pharm, Guangzhou 510080, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, Dept Neurol, Affiliated Hosp 1, Guangzhou 510080, Guangdong, Peoples R China

来源：

BIOCHEMICAL PHARMACOLOGY | 2009年 / 78卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Xyloktals; Endothelial cells; Oxidized low-density lipoprotein; Reactive oxygen species; Nitric oxide; Nicotinamide adenine dinucleotide phosphate oxidase; LOW-DENSITY-LIPOPROTEIN; HUMAN ENDOTHELIAL-CELLS; NITRIC-OXIDE; NAD(P)H OXIDASE; MAJOR SOURCE; APOPTOSIS; ACTIVATION; ATHEROSCLEROSIS; PHARMACOLOGY; INHIBITION;

D O I：

10.1016/j.bcp.2009.05.029

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Xyloketal B is a novel marine compound with unique chemical structure isolated from mangrove fungus Xylaria sp. (no. 2508). Pretreatment with xyloketal B (0.63-40 mu M) significantly improved oxLDL (150 mu g/ml)-induced injury in human umbilical vein endothelial cells (HUVECs) without either toxic or proliferative effects. Xyloketal B concentration-dependently attenuated oxLDL-induced ROS generation, peroxynitrite formation and decrease of Bcl-2 expression. In addition, xyloketal B significantly inhibited NADPH oxidase activity, as well as mRNA expression of gp91phox and p47phox. Furthermore, xyloketal B alone augmented the production of nitric oxide (NO). Collectively, these data indicate that xyloketal B protects against oxLDL-induced endothelial oxidative injury probably through inhibiting NADPH oxidase-derived ROS generation, promoting NO production and restoring Bcl-2 expression, making it a promising compound for further evaluation in the treatment of atherosclerosis. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：941 / 950

页数：10

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