Exosomes derived from pro-inflammatory bone marrow-derived mesenchymal stem cells reduce inflammation and myocardial injury via mediating macrophage polarization

被引：286

作者：

Xu, Ruqin ^{[1
]}

Zhang, Fangcheng ^{[1
]}

Chai, Renjie ^{[1
]}

Zhou, Wenyi ^{[1
]}

Hu, Ming ^{[1
]}

Liu, Bin ^{[1
]}

Chen, Xuke ^{[1
]}

Liu, Mingke ^{[1
]}

Xu, Qiong ^{[1
]}

Liu, Ningning ^{[1
]}

Liu, Shiming ^{[1
]}

机构：

[1] Guangzhou Med Univ, Affiliated Hosp 2, State Key Lab Resp Dis, Guangzhou Inst Cardiovasc Dis,Guangdong Key Lab V, Guangzhou, Guangdong, Peoples R China

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2019年 / 23卷 / 11期

基金：

中国国家自然科学基金;

关键词：

exosomes; inflammation; macrophage polarization; mesenchymal stem cells; myocardial infarction; PARTICLE-SIZE; KAPPA-B; ACTIVATION; INFARCTION; EFFICACY; THERAPY; REPAIR; TRANSPLANTATION; ROLES; M1;

D O I：

10.1111/jcmm.14635

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Exosomes are served as substitutes for stem cell therapy, playing important roles in mediating heart repair during myocardial infarction injury. Evidence have indicated that lipopolysaccharide (LPS) pre-conditioning bone marrow-derived mesenchymal stem cells (BMSCs) and their secreted exosomes promote macrophage polarization and tissue repair in several inflammation diseases; however, it has not been fully elucidated in myocardial infarction (MI). This study aimed to investigate whether LPS-primed BMSC-derived exosomes could mediate inflammation and myocardial injury via macrophage polarization after MI. Here, we found that exosomes derived from BMSCs, in both Exo and L-Exo groups, increased M2 macrophage polarization and decreased M1 macrophage polarization under LPS stimulation, which strongly depressed LPS-dependent NF-kappa B signalling pathway and partly activated the AKT1/AKT2 signalling pathway. Compared with Exo, L-Exo had superior therapeutic effects on polarizing M2 macrophage in vitro and attenuated the post-infarction inflammation and cardiomyocyte apoptosis by mediating macrophage polarization in mice MI model. Consequently, we have confidence in the perspective that low concentration of LPS pre-conditioning BMSC-derived exosomes may develop into a promising cell-free treatment strategy for clinical treatment of MI.

引用

页码：7617 / 7631

页数：15

共 60 条

[1]

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