Arabinose and xylose fermentation by recombinant Saccharomyces cerevisiae expressing a fungal pentose utilization pathway

被引:96
作者
Bettiga, Maurizio [1 ]
Bengtsson, Oskar [1 ]
Hahn-Hagerdal, Barbel [1 ]
Gorwa-Grauslund, Marie F. [1 ]
机构
[1] Lund Univ, Dept Appl Microbiol, SE-22100 Lund, Sweden
关键词
L-XYLULOSE REDUCTASE; CELL-FREE EXTRACTS; LACTOBACILLUS PLANTARUM; ETHANOL-PRODUCTION; XYLITOL-DEHYDROGENASE; POLYOL DEHYDROGENASES; ISOMERASE PATHWAYS; CATABOLIC PATHWAY; CANDIDA-ALBICANS; PICHIA-STIPITIS;
D O I
10.1186/1475-2859-8-40
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Sustainable and economically viable manufacturing of bioethanol from lignocellulose raw material is dependent on the availability of a robust ethanol producing microorganism, able to ferment all sugars present in the feedstock, including the pentose sugars L-arabinose and D-xylose. Saccharomyces cerevisiae is a robust ethanol producer, but needs to be engineered to achieve pentose sugar fermentation. Results: A new recombinant S. cerevisiae strain expressing an improved fungal pathway for the utilization of L-arabinose and D-xylose was constructed and characterized. The new strain grew aerobically on L-arabinose and D-xylose as sole carbon sources. The activities of the enzymes constituting the pentose utilization pathway(s) and product formation during anaerobic mixed sugar fermentation were characterized. Conclusion: Pentose fermenting recombinant S. cerevisiae strains were obtained by the expression of a pentose utilization pathway of entirely fungal origin. During anaerobic fermentation the strain produced biomass and ethanol. L-arabitol yield was 0.48 g per gram of consumed pentose sugar, which is considerably less than previously reported for D-xylose reductase expressing strains co-fermenting L-arabinose and D-xylose, and the xylitol yield was 0.07 g per gram of consumed pentose sugar.
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页数:12
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