Catalytic mechanism of an abzyme displaying a beta-lactamase-like activity

被引：9

作者：

Avalle, B ^{[1
]}

Débat, H ^{[1
]}

Friboulet, A ^{[1
]}

Thomas, D ^{[1
]}

机构：

[1] Univ Technol Compiegne, UPRES A 6022, F-60205 Compiegne, France

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2000年 / 83卷 / 1-3期

关键词：

beta-lactamase; antiidiotypic antibody; abzyme; catalytic antibody; suicide substrate;

D O I：

10.1385/ABAB:83:1-3:163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A catalytic IgG (Ab2) displaying a beta-lactamase-like activity was previously obtained by using the antiidiotypic pathway: the particularity of this antibody is that it is a true antiidiotype of the beta-lactamase active site. We have previously demonstrated that this IgG has retained some of the structural information displayed by the beta lactamase active site, evident from data that polyclonal anti-Ab2 antibodies (Ab3) recognize beta-lactamase. In this article, we investigated the catalytic mechanism of the abzyme compared to that of the enzyme. The experimental data allow us to hypothesize the catalytic residues required for catalysis.

引用

页码：163 / 169

页数：7

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