Increased efficiency of preimplantation genetic diagnosis for aneuploidy by testing 12 chromosomes

被引：28

作者：

Colls, P. ^{[1
]}

Goodall, N. ^{[1
]}

Zheng, X. ^{[1
]}

Munne, S. ^{[1
]}

机构：

[1] Reprogenetics LLC, Livingston, NJ 07039 USA

来源：

REPRODUCTIVE BIOMEDICINE ONLINE | 2009年 / 19卷 / 04期

关键词：

aneuploidy; FISH; pregnancy rate; preimplantation genetic diagnosis; IN-VITRO FERTILIZATION; SITU HYBRIDIZATION; FISH ANALYSIS; BLASTOMERES; POLAR BODY; EMBRYOS; ABNORMALITIES; BIOPSY; CELLS; SURVIVAL;

D O I：

10.1016/j.rbmo.2009.05.002

中图分类号：

R71 [妇产科学];

学科分类号：

100211 [妇产科学];

摘要：

One of the most important factors in increasing the screening potential of preimplantation genetic diagnosis (PGD) for aneuploidy is to increase the number of chromosomes analysed. Inclusion of chromosomes 8, 14 and 20 to the standard set of chromosomes X, Y, 13, 15, 16, 17, 18, 21 and 22 allows the analysis of 12 chromosomes in three rounds of fluorescent in-situ hybridization (FISH) without decreasing the efficiency of the technique. Pregnancy rate was significantly increased when only embryos that had been diagnosed as normal for the 12 chromosomes analysed were transferred compared with transfer of embryos with any abnormality for chromosomes 8, 14 or 20 (P < 0.05). This study proves that the high efficiency and practical feasibility of FISH analysis of 12 chromosomes in PGD for aneuploidy is a superior approach than the standard nine-chromosome analysis in order to screen for abnormalities.

引用

页码：532 / 538

页数：7

共 37 条

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[2]

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