Effects of mechanical and hydrodynamic stress in agitated, sparged cultures of Porphyridium cruentum

被引:86
作者
Camacho, FG [1 ]
Gómez, AC [1 ]
Sobczuk, TM [1 ]
Grima, EM [1 ]
机构
[1] Univ Almeria, Dept Chem Engn, Almeria 04071, Spain
关键词
fluid-mechanical stress; hydrodynamic stress; microalga; photobioreactor; Porphyridium cruentum; cell damage;
D O I
10.1016/S0032-9592(00)00138-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要
Effects of mechanical and hydrodynamic forces on cells of the microalga Porphyridium cruentum are reported for aerated cultures in stirred vessels. The specific rate of cell death varied linearly with the specific interfacial area, suggesting the importance of bubble-cell contacting in the damaging process. At low rates of mechanical agitation, bubble break-up at the culture surface was the predominant cause of damage. In contrast, at higher agitation intensities, cell damage was caused predominantly by hydrodynamic stress in bulk turbulent flow. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1045 / 1050
页数:6
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