Enumeration of Enterococcus sp. using a modified mE method

被引:10
作者
Rhodes, MW [1 ]
Kator, H [1 ]
机构
[1] VIRGINIA INST MARINE SCI, COLL WILLIAM & MARY, GLOUCESTER POINT, VA 23062 USA
关键词
D O I
10.1046/j.1365-2672.1997.00200.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A modified mE medium (mEI) containing the chromogenic substrate indoxyl-beta-D-glucoside to detect beta-D-glucosidase activity was evaluated with respect to specificity and recovery of enterococci from environmental eaters. Extending incubation from 24 to 48 h improved enterococci recovery but 77% of the colonies classified as non-target were confirmed as enterococci. Randomly chosen enterococcal isolates from sewage, exposed in microcosms containing 0.22 mu m membrane filtered fresh or estuarine water, exhibited differences in persistence as a function of exposure treatment. Decreasing the concentration of or eliminating indoxyl-beta-D-glucoside from mE did not significantly affect recovery of purified isolates.
引用
收藏
页码:120 / 126
页数:7
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