A major jasmonate-inducible protein of sweet potato, ipomoelin, is an ABA-independent wound-inducible protein

被引:30
作者
Imanishi, S [1 ]
KitoNakamura, K [1 ]
Matsuoka, K [1 ]
Morikami, A [1 ]
Nakamura, K [1 ]
机构
[1] NAGOYA UNIV, SCH AGR SCI, BIOCHEM LAB, NAGOYA, AICHI 46401, JAPAN
关键词
gene expression; ipomoelin; jasmonic acid; sporamin; sweet potato; wounding;
D O I
10.1093/oxfordjournals.pcp.a029216
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Treatment of sweet potato plants cultured in vitro with a vapor of methyl jasmonate (MeJA) induced an accumulation in leaves of a large amount of protein with an apparent molecular mass of 18 kDa, This protein, designated ipomoelin, was purified, and the amino acid sequences of proteolytic fragments were determined, Screening a cDNA library of MeJA-treated leaves by oligonucleotide probes designed from the peptide sequences identified a clone that could code for a polypeptide with 154 amino acids, The deduced amino acid sequence of ipomoelin showed an overall amino acid identity of 25% with the salt-inducible SalT protein of rice. In addition, the C-terminal 70 amino acid sequence of ipomoelin showed about 50% identity with the C-terminal amino acid sequences of seed lectins from Moraceae. The gene for ipomoelin was present in a few copies in the genome of sweet potato, The mRNA for ipomoelin was detected in leaves and petioles, but not in stems and tuberous roots, of sweet potato plants grown in the field, Mechanical wounding of leaves induced ipomoelin mRNA both locally and systemically, while treatment of leaves with ABA, salt, or a high level of sucrose did not induce ipomoelin mRNA, By contrast, ABA-inducible mRNA for sporamin was not induced by MeJA, These results suggest that ipomoelin is involved in defensive reactions of leaves in response to wounding and that JA-mediated wound-induction of ipomoelin occurs independently of ABA.
引用
收藏
页码:643 / 652
页数:10
相关论文
共 29 条
[1]   CHARACTERIZATION OF AN ARABIDOPSIS-LIPOXYGENASE GENE RESPONSIVE TO METHYL JASMONATE AND WOUNDING [J].
BELL, E ;
MULLET, JE .
PLANT PHYSIOLOGY, 1993, 103 (04) :1133-1137
[2]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3]  
BRADSTOCK M, 1989, AUST LIT STUD, V14, P51
[4]   CHARACTERIZATION OF A RICE GENE SHOWING ORGAN-SPECIFIC EXPRESSION IN RESPONSE TO SALT STRESS AND DROUGHT [J].
CLAES, B ;
DEKEYSER, R ;
VILLARROEL, R ;
VANDENBULCKE, M ;
BAUW, G ;
VANMONTAGU, M ;
CAPLAN, A .
PLANT CELL, 1990, 2 (01) :19-27
[5]   JASMONIC ACID METHYL JASMONATE ACCUMULATE IN WOUNDED SOYBEAN HYPOCOTYLS AND MODULATE WOUND GENE-EXPRESSION [J].
CREELMAN, RA ;
TIERNEY, ML ;
MULLET, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :4938-4941
[6]   STRUCTURE AND EXPRESSION OF A ROOT-SPECIFIC RICE GENE [J].
DEPATER, BS ;
SCHILPEROORT, RA .
PLANT MOLECULAR BIOLOGY, 1992, 18 (01) :161-164
[7]   OLIGOGALACTURONIDES AND CHITOSAN ACTIVATE PLANT DEFENSIVE GENES THROUGH THE OCTADECANOID PATHWAY [J].
DOARES, SH ;
SYROVETS, T ;
WEILER, EW ;
RYAN, CA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (10) :4095-4098
[8]   REGULATION OF SYNTHESIS OF PROTEINASE INHIBITOR-I AND INHIBITOR-II MESSENGER-RNAS IN LEAVES OF WOUNDED TOMATO PLANTS [J].
GRAHAM, JS ;
HALL, G ;
PEARCE, G ;
RYAN, CA .
PLANTA, 1986, 169 (03) :399-405
[9]   MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF CDNA FOR SPORAMIN, THE MAJOR SOLUBLE-PROTEIN OF SWEET-POTATO TUBEROUS ROOTS [J].
HATTORI, T ;
NAKAGAWA, T ;
MAESHIMA, M ;
NAKAMURA, K ;
ASAHI, T .
PLANT MOLECULAR BIOLOGY, 1985, 5 (05) :313-320
[10]  
HATTORI T, 1991, PLANT CELL PHYSIOL, V32, P79