Astragalus polysaccharide ameliorates H2O2-induced human umbilical vein endothelial cell injury

被引：83

作者：

Han, Ronghui ^{[1
]}

Tang, Futian ^{[1
]}

Lu, Meili ^{[1
]}

Xu, Chonghua ^{[1
]}

Hu, Jin ^{[1
]}

Mei, Meng ^{[1
]}

Wang, Hongxin ^{[2
]}

机构：

[1] Jinzhou Med Univ, Key Lab Cardiovasc & Cerebrovasc Drug Res Liaonin, Inst Drug Res, Jinzhou 121001, Liaoning, Peoples R China

[2] Jinzhou Med Univ, Key Lab Cardiovasc & Cerebrovasc Drug Res, Liaoning Drug Res Inst, Dept Pharmacol, 40 Songpo Rd, Jinzhou 121001, Liaoning, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2017年 / 15卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Astragalus polysaccharide; human umbilical vein endothelial cells; oxidative stress; nitric oxide; apoptosis; LEFT-VENTRICULAR HYPERTROPHY; INDUCED CARDIAC-HYPERTROPHY; OXIDATIVE STRESS; NITRIC-OXIDE; HYDROGEN-PEROXIDE; MITOCHONDRIAL DYSFUNCTION; CARDIOVASCULAR RISK; INDUCED APOPTOSIS; MEMBRANACEUS; MECHANISMS;

D O I：

10.3892/mmr.2017.6515

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Endothelial dysfunction caused by reactive oxygen species (ROS) has been implicated in numerous cardiovascular diseases. Astragalus polysaccharide (APS), an important bioactive component extracted from the Chinese herb Astragalus membranaceus, has been widely used for the treatment of cardiovascular disease. The present study aimed to investigate the effects of APS on hydrogen peroxide (H2O2)-induced human umbilical vein endothelial cell (HUVEC) injury. Following treatment with 400 mu M H2O2 for 24 h, cell viability was decreased and apoptosis was increased. However, pretreatment with APS for 1 h significantly attenuated H2O2-induced injury in HUVECs. In addition, APS decreased intracellular ROS levels, increased the protein expression of endothelial nitric oxide synthase and copper-zinc superoxide dismutase, elevated intracellular cyclic guanosine monophosphate (an activity marker for nitric oxide) levels and restored the mitochondrial membrane potential, compared with cells treated with H2O2 only. In conclusion, the results of the present study suggested that APS may protect HUVECs from injury induced by H2O2 via increasing the cell antioxidant capacity and nitric oxide (NO) bioavailability, which may contribute to the improvement of the imbalance between ROS and NO levels.

引用

页码：4027 / 4034

页数：8

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