tRNA maturation in Aquifex aeolicus

被引：22

作者：

Willkomm, DK ^{[1
]}

Feltens, R ^{[1
]}

Hartmann, RK ^{[1
]}

机构：

[1] Med Univ Lubeck, Inst Biochem, D-23538 Lubeck, Germany

来源：

BIOCHIMIE | 2002年 / 84卷 / 08期

关键词：

Aquifex aeolicus; tRNA processing; primer extension; RNase P; RNase E/G;

D O I：

10.1016/S0300-9084(02)01447-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several tRNAs in the hyperthermophilic bacterium Aquifex aeolicus are encoded in clusters and as part of ribosomal RNA operons, implying the requirement for tRNA processing by ribonuclease P (RNase P). Intriguingly, neither a gene for the RNA nor the protein component of this ubiquitous ribonucleoprotein enzyme has been hitherto identified in the sequenced genome of A. aeolicus, despite extensive data mining. As a result of the present study, primer extension analysis revealed that tRNAs in A. aeolicus possess canonical mature 5' ends; yet we were unable to demonstrate RNase P holoenzyme or RNase P RNA alone activity in A. aeolicus extracts under a variety of reaction conditions utilizing mono- and dimeric ptRNA substrates. Processing of dimeric ptRNA transcripts in extracts of A. aeolicus disclosed at least one endoribonuclease which cleaves in the A/U-rich spacer of the tandem ptRNA, reminiscent of bacterial RNasc E-like enzymes. (C) 2002 Societe francaise de biochimie et biologie moleculaire / Editions scientifiques et medicales Elsevier SAS. All rights reserved.

引用

页码：713 / 722

页数：10

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