Enhancement of integrins by interleukin-1α, and their relationship with metastatic and invasive behavior of human pancreatic ductal adenocarcinoma cells

被引：16

作者：

Sawai, H ^{[1
]}

Takeyama, H ^{[1
]}

Yamamoto, M ^{[1
]}

Furuta, A ^{[1
]}

Funahashi, H ^{[1
]}

Okada, Y ^{[1
]}

Sato, M ^{[1
]}

Tanaka, M ^{[1
]}

Manabe, T ^{[1
]}

机构：

[1] Nagoya City Univ, Sch Med, Dept Surg 1, Mizuho Ku, Nagoya, Aichi 4678601, Japan

来源：

JOURNAL OF SURGICAL ONCOLOGY | 2003年 / 82卷 / 01期

关键词：

adhesion molecules; integrin; interleukin-1; alpha; metastasis; pancreas cancer;

D O I：

10.1002/jso.10187

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background and Objectives: Adhesion and invasion of tumor cells to extracellular matrix (ECM) proteins play an important role in tumor metastasis formation. We investigated the enhancement of adhesive and invasive behavior to ECM proteins of human pancreatic cancer cells by interleukin-1alpha (IL-1alpha) to examine the mechanism of adhesion and invasion of metastatic human pancreatic cancer cells to ECM proteins. Methods: The enhancement of integrin subunits by IL-1alpha was examined by cellular enzyme-linked immunosorbent assay (CELISA) in two metastatic human pancreatic cancer cell lines (BxPC-3 and SW 1990) and two nonmetastatic pancreatic cancer cell lines (PaCa-2 and PANC- 1). In addition, assays of cancer cell adhesion and invasion to ECM proteins were performed to investigate whether increased integrin expression affected the invasive interaction between cancer cells and the putative integrin ECM ligands. Results: Expression of the alpha(6) subunit by metastatic cancer cells was enhanced by IL-1alpha. Metastatic cancer cells also exhibited preferential adherence and invasion to laminin compared with nonmetastatic cancer cells, and this was enhanced by IL-1alpha. Conclusions: The enhancement of alpha(6)beta(1)-integrin by Il-1alpha acting through IL-1RI, as well as the expression of alpha(6)beta(1)-integrin, plays an important role in metastasis formation in pancreatic cancer.

引用

页码：51 / 56

页数：6

共 34 条

[1]

ALBINI A, 1987, CANCER RES, V47, P3239

[2] β1 integrins play an essential role in adhesion and invasion of pancreatic carcinoma cells [J].

Arao, S ;

Masumoto, A ;

Otsuki, M .

PANCREAS, 2000, 20 (02) :129-137

[3] PROTEOLYTIC REMODELING OF EXTRACELLULAR-MATRIX [J].

BIRKEDALHANSEN, H .

CURRENT OPINION IN CELL BIOLOGY, 1995, 7 (05) :728-735

[4] HOW DOES THE EXTRACELLULAR-MATRIX DIRECT GENE-EXPRESSION [J].

BISSELL, MJ ;

HALL, HG ;

PARRY, G .

JOURNAL OF THEORETICAL BIOLOGY, 1982, 99 (01) :31-68

[5] MODULATION OF LAMININ RECEPTOR EXPRESSION BY ESTROGEN AND PROGESTINS IN HUMAN-BREAST CANCER CELL-LINES [J].

CASTRONOVO, V ;

TARABOLETTI, G ;

LIOTTA, LA ;

SOBEL, ME .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1989, 81 (10) :781-788

[6]

DADHAR S, 1996, CURR OPIN CELL BIOL, V8, P657

[7] REGULATION OF INTEGRIN-MEDIATED ADHESION TO LAMININ AND COLLAGEN IN HUMAN MELANOCYTES AND IN NONMETASTATIC AND HIGHLY METASTATIC HUMAN-MELANOMA CELLS [J].

DANEN, EHJ ;

VANMUIJEN, GNP ;

VANDEWIELVANKEMENADE, E ;

JANSEN, KFJ ;

RUITER, DJ ;

FIGDOR, CG .

INTERNATIONAL JOURNAL OF CANCER, 1993, 54 (02) :315-321

[8]

HABERERNBLOOD C, 1987, J NATL CANCER I, V79, P891

[9]

Heino J, 1996, INT J CANCER, V65, P717, DOI 10.1002/(SICI)1097-0215(19960315)65:6<717::AID-IJC1>3.0.CO

[10]

2-1

← 1 2 3 4 →