Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies Report of the BIOMED-2 Concerted Action BHM4 CT98-3936

被引:172
作者
Brueggemann, M.
White, H.
Gaulard, P.
Garcia-Sanz, R.
Gameiro, P.
Oeschger, S.
Jasani, B.
Ott, M.
Delsol, G.
Orfao, A.
Tiemann, M.
Herbst, H.
Langerak, A. W.
Spaargaren, M.
Moreau, E.
Groenen, P. J. T. A.
Sambade, C.
Foroni, L.
Carter, G. I.
Hummel, M.
Bastard, C.
Davi, F.
Delfau-Larue, M. -H.
Kneba, M.
van Dongen, J. J. M.
Beldjord, K.
Molina, T. J.
机构
[1] Univ Kiel, Med Clin 2, D-24098 Kiel, Germany
[2] Southampton Univ Hosp, NHS Trust, Wessex Immunol Serv, Mol Pathol Unit, Southampton, Hants, England
[3] CHU Henri Mondor, Dept Pathol, F-94010 Creteil, France
[4] Hosp Univ Salamanca, Serv Hematol, Salamanca, Spain
[5] Inst Portugues Oncol Francisco Gentil, Dept Hematol, Lisbon, Portugal
[6] Johann Wolfgang Goethe Univ Hosp, Dept Pathol, Frankfurt, Germany
[7] Univ Wales Coll Cardiff, Dept Pathol, Cardiff CF1 3NS, S Glam, Wales
[8] Univ Wurzburg, Inst Pathol, D-8700 Wurzburg, Germany
[9] Hosp Purpan, Anat Pathol Lab, Toulouse, France
[10] Univ Salamanca, Serv Cent Citometria, E-37008 Salamanca, Spain
[11] Univ Kiel, Inst Hamatopathol, D-24098 Kiel, Germany
[12] Univ Munster, Gerhard Domagk Inst Pathol, D-4400 Munster, Germany
[13] Univ Med Ctr Rotterdam, Erasmus MC, Dept Immunol, Rotterdam, Netherlands
[14] Univ Amsterdam, Acad Med Ctr, Dept Pathol, NL-1105 AZ Amsterdam, Netherlands
[15] H Hartziekenhuis, Lab Clin Biol, Roeselare, Belgium
[16] Radboud Univ Nijmegen Med Ctr, Dept Pathol, Nijmegen, Netherlands
[17] Med Fac Porto, Dept Pathol, Oporto, Portugal
[18] Univ Porto, Inst Mol Pathol & Immunol, Oncobiol Grp, P-4100 Oporto, Portugal
[19] Royal Free Hosp, Dept Haematol, London NW3 2QG, England
[20] City Hosp Nottingham, NHS Trust, Dept Mol Diagnost, Nottingham, England
[21] Univ Med Berlin, Charite, Inst Pathol, Berlin, Germany
[22] Ctr Henri Becquerel, Lab Genet Oncol, F-76038 Rouen, France
[23] Hop La Pitie Salpetriere, Dept Haematol, Paris, France
[24] CHU Henri Mondor, Serv Immunol Biol, F-94010 Creteil, France
[25] Hop Necker Enfants Malad, APHP, Hematol Lab, Paris, France
[26] Univ Paris 05, Paris, France
[27] Hop Hotel Dieu, Paris APHP, Dept Pathol, Paris, France
关键词
clonality; PCR; T-cell malignancies; T-cell receptor genes; TCR; BIOMED-2;
D O I
10.1038/sj.leu.2404481
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Polymerase chain reaction (PCR) assessment of clonal T-cell receptor (TCR) and immunoglobulin (Ig) gene rearrangements is an important diagnostic tool in mature T-cell neoplasms. However, lack of standardized primers and PCR protocols has hampered comparability of data in previous clonality studies. To obtain reference values for Ig/TCR rearrangement patterns, 19 European laboratories investigated 188 T-cell malignancies belonging to five World Health Organization-defined entities. The TCR/Ig spectrum of each sample was analyzed in duplicate in two different laboratories using the standardized BIOMED-2 PCR multiplex tubes accompanied by international pathology panel review. TCR clonality was detected in 99%(143/145) of all definite cases of T-cell prolymphocytic leukemia, T-cell large granular lymphocytic leukemia, peripheral T-cell lymphoma ( unspecified) and angioimmunoblastic T-cell lymphoma (AILT), whereas nine of 43 anaplastic large cell lymphomas did not show clonal TCR rearrangements. Combined use of TCRB and TCRG genes revealed two or more clonal signals in 95% of all TCR clonal cases. Ig clonality was mostly restricted to AILT. Our study indicates that the BIOMED-2 multiplex PCR tubes provide a powerful strategy for clonality assessment in T-cell malignancies assisting the firm diagnosis of T-cell neoplasms. The detected TCR gene rearrangements can also be used as PCR targets for monitoring of minimal residual disease.
引用
收藏
页码:215 / 221
页数:7
相关论文
共 51 条
[1]   B-CELL LYMPHOMA AFTER ANGIOIMMUNOBLASTIC LYMPHADENOPATHY - A CASE WITH OLIGOCLONAL GENE REARRANGEMENTS ASSOCIATED WITH EPSTEIN-BARR-VIRUS [J].
ABRUZZO, LV ;
SCHMIDT, K ;
WEISS, LM ;
JAFFE, ES ;
MEDEIROS, LJ ;
SANDER, CA ;
RAFFELD, M .
BLOOD, 1993, 82 (01) :241-246
[2]   Analysis of TCR, pTα, and RAG-1 in T-acute lymphoblastic leukemias improves understanding of early human T-lymphoid lineage commitment [J].
Asnafi, V ;
Beldjord, K ;
Boulanger, E ;
Comba, B ;
Le Tutour, P ;
Estienne, MH ;
Davi, F ;
Landman-Parker, J ;
Quartier, P ;
Buzyn, A ;
Delabesse, E ;
Valensi, F ;
Macintyre, E .
BLOOD, 2003, 101 (07) :2693-2703
[3]  
Assaf C, 2000, BLOOD, V96, P640
[4]   Neoplastic T cells in angioimmunoblastic T-cell lymphoma express CD10 [J].
Attygalle, A ;
Al-Jehani, R ;
Diss, TC ;
Munson, P ;
Liu, HX ;
Du, MQ ;
Isaacson, PG ;
Dogan, A .
BLOOD, 2002, 99 (02) :627-633
[5]  
Blom B, 1999, BLOOD, V93, P3033
[6]   Anaplastic large cell lymphomas lack the expression of T-cell receptor molecules or molecules of proximal T-cell receptor signaling [J].
Bonzheim, I ;
Geissinger, E ;
Roth, S ;
Zettl, A ;
Marx, A ;
Rosenwald, A ;
Müller-Hermelink, HK ;
Rüdiger, T .
BLOOD, 2004, 104 (10) :3358-3360
[7]   HETERODUPLEX ANALYSIS OF T-CELL RECEPTOR-GAMMA GENE REARRANGEMENTS FOR DIAGNOSIS AND MONITORING OF CUTANEOUS T-CELL LYMPHOMAS [J].
BOTTARO, M ;
BERTI, E ;
BIONDI, A ;
MIGONE, N ;
CROSTI, L .
BLOOD, 1994, 83 (11) :3271-3278
[8]   Survival and clonal expansion of mutating "forbidden" (immunoglobulin receptor-deficient) Epstein-Barr virus-infected B cells in angioimmunoblastic T cell lymphoma [J].
Bräuninger, A ;
Spieker, T ;
Willenbrock, K ;
Gaulard, P ;
Wacker, HH ;
Rajewsky, K ;
Hansmann, ML ;
Küppers, R .
JOURNAL OF EXPERIMENTAL MEDICINE, 2001, 194 (07) :927-940
[9]  
BREIT TM, 1994, J IMMUNOL, V152, P2860
[10]  
BREIT TM, 1993, BLOOD, V82, P3063