Interaction of sulfur mustard with rat liver salt fractionated chromatin

被引：11

作者：

Jafari, Mahvash ^{[1
]}

Nateghi, M. ^{[2
]}

Rabbani, A. ^{[3
]}

机构：

[1] Baqiyatallah Univ Med Sci, Dept Biochem, Fac Med, Chem Injuries Res Ctr, Tehran, Iran

[2] Baqiyatallah Univ Med Sci, Res Ctr Mol Biol, Tehran, Iran

[3] Univ Tehran, IBB, Tehran, Iran

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2010年 / 46卷 / 01期

关键词：

Sulfur mustard; Active and inactive chromatin; Rat liver; TRANSCRIPTIONALLY ACTIVE CHROMATIN; YOSHIDA ASCITES SARCOMA; DNA CROSS-LINKING; NUCLEAR PROTEINS; ENDOTHELIAL-CELLS; CHEMICAL WARFARE; APOPTOSIS; KERATINOCYTES; CHLORAMBUCIL; AGENTS;

D O I：

10.1016/j.ijbiomac.2009.10.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In this study, the interaction of an alkylating agent, sulfur mustard (SM) with rat liver active (S(1) and S(2)) and inactive (P(2)) chromatin was investigated employing UV/vis spectroscopy and gel electrophoreses. The results show that SM affects the chromatin structure in a dose-dependent manner. The binding of SM to fractions is different. At lower concentrations (<500 mu M), SM seems to unfold the structure and at higher concentrations, it induces aggregation and condensation of chromatin possibly via forming cross-links between the chromatin components. The extent of condensation in S(2) is higher when compared to the P(2) fraction. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：104 / 108

页数：5

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