STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins

被引：59

作者：

Fitzpatrick, James A. J. ^{[1
]}

Yan, Qi ^{[1
]}

Sieber, Jochen J. ^{[1
]}

Dyba, Marcus ^{[1
]}

Schwarz, Ulf ^{[1
]}

Szent-Gyorgyi, Chris ^{[1
]}

Woolford, Carol A. ^{[1
]}

Berget, Peter B. ^{[1
]}

Waggoner, Alan S. ^{[1
]}

Bruchez, Marcel P. ^{[1
]}

机构：

[1] Carnegie Mellon Univ, Mol Biosensor & Imaging Ctr, Dept Chem, Pittsburgh, PA 15213 USA

来源：

BIOCONJUGATE CHEMISTRY | 2009年 / 20卷 / 10期

基金：

美国安德鲁·梅隆基金会;

关键词：

STRUCTURED-ILLUMINATION MICROSCOPY; FIELD OPTICAL NANOSCOPY; LOCALIZATION MICROSCOPY; STIMULATED-EMISSION; RESOLUTION LIMIT; FLUORESCENCE; DYES;

D O I：

10.1021/bc900249e

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate the effectiveness of a genetically encoded malachite green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expressed FAP or as an intracellular FAP-actin fusion. Structures with widths of 110-122 nm were observed. Depletion data, however, suggest a resolution of 70 nm with the given instrument.

引用

页码：1843 / 1847

页数：5

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