Dietary enrichment with omega-3 polyunsaturated fatty acids reverses age-related decreases in the GluR2 and NR2B glutamate receptor subunits in rat forebrain

被引:90
作者
Dyall, S. C.
Michael, G. J.
Whelpton, R.
Scott, A. G.
Michael-Titus, A. T.
机构
[1] Univ London, St Bartholomews & Royal London Sch Med & Dent, Neurosci Ctr, London E1 2AT, England
[2] Queen Mary Univ London, London E1 4NS, England
关键词
docosahexaenoic acid; eicosapentaenoic acid; glutamate receptors; phospholipids; rat; ageing; prefrontal cortex; striatum; hippocampus; omega-3 fatty acids;
D O I
10.1016/j.neurobiolaging.2006.01.002
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
Ageing is associated with a decrease in the brain content of omega-3 polyunsaturated fatty acids (PUFA), such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and with decreased neuroplasticity. The glutamate receptor subunits GluR2 and NR2B play a significant role in forebrain synaptic plasticity. We investigated GluR2 and NR2B in the aged prefrontal cortex, hippocampus and striatum, and tested if treatment with a preparation containing EPA and DHA can reverse age-related changes. The study compared adult and old (3-4 and 24-26 month) rats, and the latter were fed a standard diet or a diet supplemented for 12 weeks with omega-3 PUFA at 270 mg/kg/day (ratio EPA to DHA 1.5:1). Ageing was associated with decreases in the GluR2 and NR2B subunits in all structures. These decreases were fully reversed by omega-3 PUFA supplementation. Age-related changes in the phospholipid PUFA content were also seen. Decreases in DHA were mostly corrected by supplementation. This study supports the neuroprotective effect of omega-3 fatty acids in brain ageing, and illustrates specific mechanisms underlying this effect. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:424 / 439
页数:16
相关论文
共 70 条
[1]   Phylogenetic analysis reveals that a dwarfing disease on different cereal crops in China is due to rice black streaked dwarf virus (RBSDV) [J].
Bai, FW ;
Yan, J ;
Qu, ZC ;
Zhang, HW ;
Xu, J ;
Ye, MM ;
Shen, DL .
VIRUS GENES, 2002, 25 (02) :201-206
[2]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF PHOSPHOLIPIDS FROM DIFFERENT SOURCES WITH COMBINED FLUORESCENCE AND ULTRAVIOLET DETECTION [J].
BERNHARD, W ;
LINCK, M ;
CREUTZBURG, H ;
POSTLE, AD ;
ARNING, A ;
MARTINCARRERA, I ;
SEWING, KF .
ANALYTICAL BIOCHEMISTRY, 1994, 220 (01) :172-180
[3]   DELTA-6 DESATURASE IN BRAIN AND LIVER DURING DEVELOPMENT AND AGING [J].
BOURRE, JM ;
PICIOTTI, M ;
DUMONT, O .
LIPIDS, 1990, 25 (06) :354-356
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   Dietary n-3 polyunsaturated fatty acid depletion activates caspases and decreases NMDA receptors in the brain of a transgenic mouse model of Alzheimer's disease [J].
Calon, F ;
Lim, GP ;
Morihara, T ;
Yang, FS ;
Ubeda, O ;
Salem, N ;
Frautschy, SA ;
Cole, GM .
EUROPEAN JOURNAL OF NEUROSCIENCE, 2005, 22 (03) :617-626
[6]  
Carrié I, 2000, J LIPID RES, V41, P465
[7]   The relationship between age and the fatty acid composition of cerebral cortex and erythrocytes in human subjects [J].
Carver, JD ;
Benford, VJ ;
Han, B ;
Cantor, AB .
BRAIN RESEARCH BULLETIN, 2001, 56 (02) :79-85
[8]   AGE-DEPENDENT LOSS OF NMDA RECEPTORS IN HIPPOCAMPUS, STRIATUM, AND FRONTAL-CORTEX OF THE RAT - PREVENTION BY ACETYL-L-CARNITINE [J].
CASTORINA, M ;
AMBROSINI, AM ;
PACIFICI, L ;
RAMACCI, MT ;
ANGELUCCI, L .
NEUROCHEMICAL RESEARCH, 1994, 19 (07) :795-798
[9]   Deficits in the expression of the NR2B subunit in the hippocampus of aged Fisher 344 rats [J].
Clayton, DA ;
Browning, MD .
NEUROBIOLOGY OF AGING, 2001, 22 (01) :165-168
[10]   AGE-RELATED ALTERATIONS OF NMDA-RECEPTOR PROPERTIES IN THE MOUSE FOREBRAIN - PARTIAL RESTORATION BY CHRONIC PHOSPHATIDYLSERINE TREATMENT [J].
COHEN, SA ;
MULLER, WE .
BRAIN RESEARCH, 1992, 584 (1-2) :174-180