Molecular characterization of enteroviruses isolated from patients with aseptic meningitis in Korea, 2005

被引:21
作者
Lee, S.-T. [1 ]
Ki, C.-S. [1 ]
Lee, N. Y. [1 ]
机构
[1] Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Lab Med, Seoul 135710, South Korea
关键词
D O I
10.1007/s00705-006-0901-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Enteroviruses are the most common causative agents of aseptic meningitis. In 2005, an outbreak of aseptic meningitis occurred in Seoul, Korea, which appeared to be caused by certain enterovirus strains. This study analyzed the clinical isolates from 24 different aseptic meningitis patients at our center from January to September 2005. Serotyping using type-specific antisera, RT-PCR, and direct sequencing of the 5' noncoding and VP1 regions were carried out. The serotyping study identified 15 coxsackievirus B5 (CBV5), 4 coxsackievirus B1 (CBV1), 1 coxsackievirus B3 (CBV3) and 4 echovirus 9 (ECV9). Phylogenic analysis of the VP1 region revealed CBV5 to be the most predominant strain (15 strains). Twelve of these strains were grouped together with recent strains isolated in China and France. The 12 CBV5 strains contained a novel amino acid change in the BC-loop (the 90th and 91st, AY -> GN) compared to the reference strain (1954/UK/85). The amino acid changes in the BC-loop in some recent CBV5 strains tend to be located in the C-terminal part, and these changes suggest some antigenic significance.
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收藏
页码:963 / 970
页数:8
相关论文
共 17 条
[1]   Improved genotyping vaccine and wild-type poliovirus strains by restriction fragment length polymorphism analysis: Clinical diagnostic implications [J].
Georgopoulou, A ;
Markoulatos, P ;
Spyrou, N ;
Vamvakopoulos, NC .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (12) :4337-4342
[2]   REEMERGENCE OF AN EPIDEMIC COXSACKIEVIRUS B5 GENOTYPE [J].
HAMBY, BB ;
PALLANSCH, MA ;
KEW, OM .
JOURNAL OF INFECTIOUS DISEASES, 1987, 156 (02) :288-292
[3]   Molecular biological characterization of enterovirus variant isolated from patients with aseptic meningitis [J].
Jung, YT ;
Kim, GR ;
Paik, SY .
EXPERIMENTAL AND MOLECULAR MEDICINE, 1998, 30 (02) :101-107
[4]   GENOTYPIC VARIATION IN COXSACKIEVIRUS-B5 ISOLATES FROM 3 DIFFERENT OUTBREAKS IN THE UNITED-STATES [J].
KOPECKA, H ;
BROWN, B ;
PALLANSCH, M .
VIRUS RESEARCH, 1995, 38 (2-3) :125-136
[5]   Molecular analysis of the prototype coxsackievirus B5 genome [J].
Lindberg, AM ;
Polacek, C .
ARCHIVES OF VIROLOGY, 2000, 145 (02) :205-221
[6]   ANTIBODY RECOGNITION OF PICORNAVIRUSES AND ESCAPE FROM NEUTRALIZATION - A STRUCTURAL VIEW [J].
MATEU, MG .
VIRUS RESEARCH, 1995, 38 (01) :1-24
[7]   CHARACTERIZATION OF THE N-TERMINAL PART OF THE NEUTRALIZING ANTIGENIC SITE-I OF COXSACKIEVIRUS B4 BY MUTATION ANALYSIS OF ANTIGEN CHIMERAS [J].
MCPHEE, F ;
ZELL, R ;
REIMANN, BY ;
HOFSCHNEIDER, PH ;
KANDOLF, R .
VIRUS RESEARCH, 1994, 34 (02) :139-151
[8]  
Minor P. D., 2004, PRINCIPLES PRACTICE
[9]   Molecular typing of enteroviruses:: Current status and future requirements [J].
Muir, P ;
Kämmerer, U ;
Korn, K ;
Mulders, MN ;
Pöyry, T ;
Weissbrich, B ;
Kandolf, R ;
Cleator, GM ;
van Loon, AM .
CLINICAL MICROBIOLOGY REVIEWS, 1998, 11 (01) :202-+
[10]   Sequencing of 'untypable' enteroviruses reveals two new types, EV-77 and EV-78, within human enterovirus type B and substitutions in the BC loop of the VP1 protein for known types [J].
Norder, H ;
Bjerregaard, L ;
Magnius, L ;
Lina, B ;
Ayrnard, M ;
Chomel, JJ .
JOURNAL OF GENERAL VIROLOGY, 2003, 84 :827-836