Liquid chromatography - electrochemical detector for the determination of glucose in rat brain combined with in vivo microdialysis

被引：41

作者：

Shi, GY

Lu, JX

Xu, F

Zhou, HG

Jin, LT ^{[1
]}

Jin, JY

机构：

[1] E China Normal Univ, Sch Chem & Biol, Dept Chem, Shanghai 200062, Peoples R China

[2] Gifu Univ, Fac Engn, Dept Chem, Gifu 50111, Japan

来源：

ANALYTICA CHIMICA ACTA | 2000年 / 413卷 / 1-2期

基金：

上海市自然科学基金;

关键词：

glucose; CoTAPC/GOD/Nafion biosensor; LC-ECD; microdialysis sampling;

D O I：

10.1016/S0003-2670(00)00753-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, microdialysis sampling was combined with liquid chromatography with electrochemical detection (LC-ECD) and glucose in rat brain was determined. A cobalt tetraaminophthalocyanine (CoTAPC)/glucose oxidase (GOD)/Nafion film-coated glassy carbon electrode was used to selectively detect the hydrogen peroxide generated enzymatically in the enzyme reactors, without any interference from electroactive species such as L-ascorbate, mate, proteins. The signal current was linearly related to the glucose concentration in the range of 1.0x10(-6)-5.0x10(-3) mol/l at +0.5 V (versus Ag/AgCl) with a detection limit of 5.0x10(-7) mol/l. To measure glucose in rat brain, microdialysis sampling was employed. The dialysate from the microdialysis system was injected into system. The result was 0.74+/-0.10 mmol/l (n=4). (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：131 / 136

页数：6

共 18 条

[1] SYNTHESIS AND STRUCTURAL STUDIES OF METAL(II) 4,9,16,23-PHTHALOCYANINE TETRAAMINES [J].