Monoterpene synthase activities in leaves of Picea abies (L.) Karst. and Quercus ilex L.

被引:65
作者
Fischbach, RJ [1 ]
Zimmer, I [1 ]
Steinbrecher, R [1 ]
Pfichner, A [1 ]
Schnitzler, JP [1 ]
机构
[1] Fraunhofer Inst Atmosphar Umweltforsch, D-82467 Garmisch Partenkirchen, Germany
关键词
Picea abies; Pinaceae; Quercus ilex; Fagaceae; evergreen leaves; enzyme; characterization; monoterpene synthases;
D O I
10.1016/S0031-9422(00)00119-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In addition to direct ecological functions in the interaction of plants with the environment, the emission of monoterpenes, especially from the foliage of evergreen trees, is of great importance for the production of ozone and photochemical oxidants in the troposphere. In the present work, we established a reproducible non-radioactive standard enzyme assay and characterized monoterpene synthase activities in needles of Norway spruce (Picea abies (L.) Karst.) and in leaves of helm oak (Quercus ilex L.). In Norway spruce, the dominant monoterpenes formed were alpha-pinene, camphene, and to a lesser extent beta-pinene and limonene. In helm oak. alpha-pinene, sabinene, and beta-pinene were the main products. while limonene was a minor component. Under optimum conditions, in both Norway spruce and helm oak, monoterpene formation remained constant up to 180 min and 90 min. respectively, and varied with the buffer and Mg2+ and Mn2+ concentrations used. Optimum temperature for monoterpene synthase activity was 40 degrees C in both species: optimal pH ranged between 6.5 and 7.5 in both species. Apparent Michaelis-constants for the substrate GDP were ca. 17.9 +/- 5.1 mu M for Norway spruce and ca. 69.4 +/- 22.1 mu M for holm oak. Molecular weight determination by FPLC indicated that the monoterpene synthases in Norway spruce and helm oak have native molecular weights of ca. 59 and 50 kDa, respectively. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:257 / 265
页数:9
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