Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system

被引：58

作者：

Borowski, H. ^{[1
]}

Thompson, R. C. A. ^{[1
]}

Armstrong, T. ^{[1
]}

Clode, P. L. ^{[2
]}

机构：

[1] Murdoch Univ, WHO Collaborating Ctr Mol Epidemiol Parasit Infec, Murdoch, WA 6150, Australia

[2] Univ Western Australia, Ctr Microscopy Characterisat & Anal, Crawley, WA 6009, Australia

来源：

PARASITOLOGY | 2010年 / 137卷 / 01期

关键词：

Cryptosporidium parvum; morphology; host cell interaction; phylogenetic affinity; gregarines; electron microscopy; HOST-CELL MEMBRANE; INVASION; ATTACHMENT; SPOROZOITE; PROTEIN; INTERNALIZATION; APICOMPLEXA; EXCYSTATION; COCCIDIA; GAMONTS;

D O I：

10.1017/S0031182009990837

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程]; 100103 [病原生物学];

摘要：

Cryptosporidium parvum is a zoonotic protozoan parasite that mainly affects the ileum of humans and livestock, with the potential to cause severe enteric disease. We describe the complete life cycle of C. parvum in an in vitro system. Infected cultures of the human ileocecal epithelial cell line (HCT-8) were observed over time using electron microscopy. Additional data are presented on the morphology, development and behavioural characteristics of the different life-cycle stages as well as determining their time of occurrence after inoculation. Numerous stages of C. parvum and their behaviour have been visualized and morphologically characterized for the first time using scanning electron microscopy. Further, parasite-host interactions and the effect of C. parvum on host cells were also visualized. An improved understanding of the parasite's biology, proliferation and interactions with host cells will aid in the development of treatments for the disease.

引用

页码：13 / 26

页数：14

共 36 条

[1]

A novel multi-domain mucin-like glycoprotein of Cryptosporidium parvum mediates invasion [J].