X-ray radiation-induced damage in DNA monitored by online Raman

被引：40

作者：

McGeehan, John E.

Carpentier, Philippe

Royant, Antoine

Bourgeois, Dominique

Ravelli, Raimond B. G.

机构：

[1] European Mol Biol Lab, F-38042 Grenoble 9, France

[2] Inst Biol Struct, CNRS, CEA, UFJ,UMR 5075, F-38027 Grenoble, France

[3] European Synchrotron Radiat Facil, F-38043 Grenoble, France

来源：

JOURNAL OF SYNCHROTRON RADIATION | 2007年 / 14卷

关键词：

radiation damage; radiation-damage-induced phasing; Raman spectroscopy; DNA;

D O I：

10.1107/S0909049506043251

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

High-quality Raman spectra have been collected on single DNA crystals kept at 100 K in a flow of cold nitrogen gas prior, during and after macromolecular crystallography (MX) data collection. The non-symmetric bending vibration for C-Br bonds in 8-bromo-2'-deoxyguanosine has been unambiguously identified at 293 cm(-1) in the non-resonant Raman spectra. The breakage of this bond could be monitored by the continuous recording of Raman spectra during X-ray exposure, and its decay versus dose could be directly correlated with the loss of the bromide definition within the electron-density maps as determined with MX.

引用

收藏

页码：99 / 108

页数：10

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