Bacterial assemblage structure and carbon metabolism along a productivity gradient in the NE Atlantic Ocean

被引:66
作者
Alonso-Saez, Laura
Aristegui, Javier
Pinhassi, Jarone
Gomez-Consarnau, Laura
Gonzalez, Jose M.
Vaque, Dolors
Agusti, Susana
Gasol, Josep M.
机构
[1] CSIC, Inst Ciencies Mar, Dept Biol Marina & Oceanog, Barcelona 08003, Catalunya, Spain
[2] Univ Palmas Gran Canaria, Dept Biol, Las Palmas Gran Canaria 35017, Spain
[3] Univ Kalmar, Dept Biol & environm Sci, Kalmar, Sweden
[4] Univ La Laguna, Fac Farm, Dept Microbiol & Biol Celular, San Cristobal la Laguna 38071, Spain
[5] Univ Illes Balears, CSIC, IMEDEA, Esporles, Mallorca, Spain
关键词
bacteria; Atlantic Ocean; diversity; metabolism; CARD-FISH; DGGE; IN-SITU HYBRIDIZATION; DOMINANT BACTERIOPLANKTON GROUPS; TARGETED OLIGONUCLEOTIDE PROBES; CATALYZED REPORTER DEPOSITION; PELAGIC MARINE-BACTERIA; NUCLEIC-ACID CONTENT; COMMUNITY COMPOSITION; GEL-ELECTROPHORESIS; SPATIAL VARIABILITY; COASTAL WATERS;
D O I
10.3354/ame046043
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Bacterioplankton have the potential to significantly affect the cycling of organic matter in the ocean; however, little is known about the linkage between bacterial assemblage structure and carbon metabolism. In this study, we investigated whether changes in the phylogenetic composition of bacterioplankton were associated with changes in bacterial carbon processing (bacterial production, respiration and biomass) in the subtropical NE Atlantic Ocean. We found consistent differences in the composition of the bacterial assemblage, as revealed by denaturing gradient gel electrophoresis (DGGE) and catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH), along a gradient from the NW African upwelling to the oligotrophic North Atlantic Subtropical Gyre. The percent contribution of Bacteroidetes, Roseobacter and Gammaproteobacteria significantly increased towards more productive waters, whereas the SAR11 clade of the Alphaproteobacteria remained relatively constant (average 28% of DAPI-stained cells) throughout the area. Changes in the composition of the bacterial assemblage detected by DGGE were weakly but significantly correlated with changes in carbon processing variables. The abundances of Roseobacter and Gammaproteobacteria were highly correlated with the concentration of particulate organic carbon and chlorophyll a, reflecting the affinity of these groups to nutrient-enriched conditions. The abundance of Roseobacter was also positively correlated with heterotrophic bacterial production, suggesting their active participation in carbon processing.
引用
收藏
页码:43 / 53
页数:11
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