Artificial reporter gene providing MRI contrast based on proton exchange

被引：287

作者：

Gilad, Assaf A.

McMahon, Michael T.

Walczak, Piotr

Winnard, Paul T., Jr.

Raman, Venu

van Laarhoven, Hanneke W. M.

Skoglund, Cynthia M.

Bulte, Jeff W. M.

van Zijl, Peter C. M.

机构：

[1] Russell H Morgan Dept Radiol & Radiol Sci, Neurosect, Div MR Res, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Inst Cell Engn & Oncol, Baltimore, MD 21218 USA

[3] Kennedy Krieger Inst, FM Kirby Ctr Funct Brain Imaging, Baltimore, MD 21205 USA

[4] Radboud Univ Nijmegen, Ctr Med, Dept Med Oncol 452, NL-6500 HB Nijmegen, Netherlands

来源：

NATURE BIOTECHNOLOGY | 2007年 / 25卷 / 02期

关键词：

D O I：

10.1038/nbt1277

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Existing magnetic resonance reporter genes all rely on the presence of ( super) paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein ( LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.

引用

收藏

页码：217 / 219

页数：3

相关论文

共 15 条

[1] Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells [J].

Bulte, JWM ;

Douglas, T ;

Witwer, B ;

Zhang, SC ;

Strable, E ;

Lewis, BK ;

Zywicke, H ;

Miller, B ;

van Gelderen, P ;

Moskowitz, BM ;

Duncan, ID ;

Frank, JA .

NATURE BIOTECHNOLOGY, 2001, 19 (12) :1141-1147

[2] Ferritin as an endogenous MRI reporter for noninvasive imaging of gene expression in C6 glioma tumors [J].

Cohen, B ;

Dafni, H ;

Meir, G ;

Harmelin, A ;

Neeman, M .

NEOPLASIA, 2005, 7 (02) :109-117

[3]

Genove G, 2005, NAT MED, V11, P450, DOI 10.1038/nm1208

[4] Sensitive NMR detection of cationic-polymer-based gene delivery systems using saturation transfer via proton exchange [J].

Goffeney, N ;

Bulte, JWM ;

Duyn, J ;

Bryant, LH ;

van Zijl, PCM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (35) :8628-8629

[5] Amide proton transfer imaging of human brain tumors at 3T [J].

Jones, Craig K. ;

Schlosser, Michael J. ;

van Zijl, Peter C. M. ;

Pomper, Martin G. ;

Golay, Xavier ;

Zhou, Jinyuan .

MAGNETIC RESONANCE IN MEDICINE, 2006, 56 (03) :585-592

[6] In vivo visualization of gene expression using magnetic resonance imaging [J].

Louie, AY ;

Hüber, MM ;

Ahrens, ET ;

Rothbächer, U ;

Moats, R ;

Jacobs, RE ;

Fraser, SE ;

Meade, TJ .

NATURE BIOTECHNOLOGY, 2000, 18 (03) :321-325

[7] Quantifying exchange rates in chemical exchange saturation transfer agents using the saturation time and saturation power dependencies of the magnetization transfer effect on the magnetic resonance imaging signal (QUEST and QUESP): pH calibration for poly-L-lysine and a starburst dendrimer [J].

McMahon, MT ;

Gilad, AA ;

Zhou, JY ;

Sun, PZ ;

Bulte, JWM ;

van Zijl, PCM .

MAGNETIC RESONANCE IN MEDICINE, 2006, 55 (04) :836-847

[8] Mapping transplanted stem cell migration after a stroke: a serial, in vivo magnetic resonance imaging study [J].

Modo, M ;

Mellodew, K ;

Cash, D ;

Fraser, SE ;

Meade, TJ ;

Price, J ;

Williams, SCR .

NEUROIMAGE, 2004, 21 (01) :311-317

[9] Ln(III)-DOTAMGIY complexes: A versatile series to assess the determinants of the efficacy of paramagnetic chemical exchange saturation transfer agents for magnetic resonance imaging applications [J].

Terreno, E ;

Delli Castelli, D ;

Cravotto, G ;

Milone, L ;

Aime, S .

INVESTIGATIVE RADIOLOGY, 2004, 39 (04) :235-243

[10] A new class of contrast agents for MRI based on proton chemical exchange dependent saturation transfer (CEST) [J].

Ward, KM ;

Aletras, AH ;

Balaban, RS .

JOURNAL OF MAGNETIC RESONANCE, 2000, 143 (01) :79-87