Intracellular calcium responses in CF/T43 cells: calcium pools and influx pathways

被引:4
作者
Harris, RA
Pon, DJ
Katz, S
Hanrahan, JW
机构
[1] UNIV BRITISH COLUMBIA, RESP HLTH NETWORK CTR EXCELLENCE, VANCOUVER, BC V6T 1Z3, CANADA
[2] MERCK FROSST CTR THERAPEUT RES, DEPT PHARMACOL, KIRKLAND, PQ H9H 3L1, CANADA
[3] MCGILL UNIV, DEPT PHYSIOL, MONTREAL, PQ H3G 1Y6, CANADA
[4] MCGILL UNIV, HLTH NEWTORK CTR EXCELLENCE, MONTREAL, PQ H3G 1Y6, CANADA
关键词
second messenger; airway epithelia; fura; 2; bradykinin; histamine; intracellular calcium;
D O I
10.1152/ajplung.1997.273.2.L363
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
We studied the intracellular free Ca2+ concentration ([Ca2+](i)) response to histamine in a cystic fibrosis airway epithelial cell line (CF/T43). Histamine (100 mu M; duration similar to 10 min) biphasically increased [Ca2+](i), with a rapid initial peak (30-45 s) followed by a smaller second peak that lasted for several minutes before returning to baseline. Neither peak specifically depended on Ca2+ influx. Exposure to bradykinin (10 mu M) elicited a single peak that lasted 3-3.5 min before returning to baseline. Bradykinin increased intracellular inositol 1,4,5-trisphosphate (IP3), which peaked and returned to baseline within 150 s. Histamine also increased IP3 monophasically, but the peak was brief(<20 s). Both phases of the Ca2+ response to histamine exhibited similar responsiveness to histamine concentration and sensitivity to antagonists. Cimetidine or thioperamide (1 mM) had no effect on the second peak. Pyrilamine blocked the second peak at concentrations similar to those required to block the initial peak. Activation of the second peak was observed at a threshold concentration of 1 mu M comparable with the threshold of the initial peak. Neither adenosine 3',5'-cyclic monophosphate, guanosine 3',5'-cyclic monophosphate, nor cyclic ADP (cADP) ribose altered the second phase of the histamine response.
引用
收藏
页码:L363 / L373
页数:11
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