Glycosaminoglycans affect the action of human plasma kallikrein on kininogen hydrolysis and inflammation

被引:29
作者
Andrezza, JG
Nunes, VA
Carmona, AK
Nader, HB
von Dietrich, CP
Silveira, VLF
Shimamoto, K
Ura, N
Sampaio, MU
Sampaio, CAM
Araújo, MS
机构
[1] Univ Fed Sao Paulo, Dept Biochem, EPM, Sao Paulo, Brazil
[2] Univ Fed Sao Paulo, Dept Biophys, EPM, Sao Paulo, Brazil
[3] Univ Fed Sao Paulo, Dept Physiol, EPM, Sao Paulo, Brazil
[4] Sapporo Med Univ, Sapporo, Hokkaido, Japan
基金
巴西圣保罗研究基金会;
关键词
human plasma kallikrein; high-molecular-weight kininogen; bradykinin; glycosaminoglycans; paw edema;
D O I
10.1016/S1567-5769(02)00145-5
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Human plasma kallikrein (huPK) is a serine proteinase involved in many biological processes including those of the kallikrein-kinin system. The action of huPK on kininogen results in bradykinin (BK) release, a potent mediator of inflammatory responses. BK generation may be influenced by several agents, and the aim of this work was to investigate the effect of glycosaminoglycans (GAGs) on human high-molecular-weight kininogen (HK) hydrolysis by huPK and on inflammation. huPK was pre-incubated in the absence and presence of different GAGs, followed by the addition of kininogen. Bradykinin released at different times was measured by radioimmunoassay, and K-M and k(cat) were calculated. Tuna and bovine dermatan sulfates, the most potent GAGs studied, reduced by 80% and 68%. respectively, the catalytic efficiency of huPK (control=4.1 x 10(4) M-1 s(-1)) in BK release. The effect of bovine dermatan sulfate (BDS) on inflammatory response was studied in rat paw edema induced by carrageenin and hourly determined (1-4 h) by plethysmography. BDS significantly reduced the inflammatory response in the first and second hours of measurements (24% and 28%, respectively), p < 0.05. GAGs were shown to reduce bradykinin release "in vitro" and in an inflammation model. This reduction may play a role in the control or maintenance of some pathological and physiological processes. (C) 2002 Published by Elsevier Science B.V.
引用
收藏
页码:1861 / 1865
页数:5
相关论文
共 24 条
[1]   Caiman crocodilus yacare plasma kininogen detection [J].
Araujo, MS ;
Andreotti, R ;
Tiaen, M ;
Nunes, V ;
Oliva, ML ;
Sampaio, M ;
Iimura, O ;
Shimamoto, K ;
Ura, N ;
Sampaio, C .
IMMUNOPHARMACOLOGY, 1996, 32 (1-3) :82-84
[2]   Effects of a nonpeptide bradykinin B-2 receptor antagonist, FR167344, on different in vivo animal models of inflammation [J].
Asano, M ;
Hatori, C ;
Inamura, N ;
Sawai, H ;
Hirosumi, J ;
Fujiwara, T ;
Nakahara, K .
BRITISH JOURNAL OF PHARMACOLOGY, 1997, 122 (07) :1436-1440
[3]  
Brunnee T, 1997, CLIN EXP ALLERGY, V27, P653
[4]   Contact system: A vascular biology modulator with anticoagulant, Profibrinolytic, antiadhesive, and proinflammatory attributes [J].
Colman, RW ;
Schmaier, AH .
BLOOD, 1997, 90 (10) :3819-3843
[5]   KININS AND PERITONEAL EXUDATES INDUCED BY CARRAGEENAN AND ZYMOSAN IN RATS [J].
DAMAS, J ;
BOURDON, V ;
REMACLEVOLON, G ;
ADAM, A .
BRITISH JOURNAL OF PHARMACOLOGY, 1990, 101 (02) :418-422
[6]   Pathways for bradykinin formation and inflammatory disease [J].
Kaplan, AP ;
Joseph, K ;
Silverberg, M .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2002, 109 (02) :195-209
[7]   Kinetic analysis of the role of zinc in the interaction of domain 5 of high-molecular weight kininogen (HK) with heparin [J].
Lin, YZ ;
Pixley, RA ;
Colman, RW .
BIOCHEMISTRY, 2000, 39 (17) :5104-5110
[8]   IDENTIFICATION OF PREKALLIKREIN AND HIGH-MOLECULAR-WEIGHT KININOGEN AS A COMPLEX IN HUMAN-PLASMA [J].
MANDLE, RJ ;
COLMAN, RW ;
KAPLAN, AP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (11) :4179-4183
[9]   Influence of low molecular weight heparin and low molecular weight dextran sulfate on the inhibition of coagulation factor XIa by serpins [J].
Mauron, T ;
Lämmle, B ;
Wuillemin, WA .
THROMBOSIS AND HAEMOSTASIS, 1998, 80 (01) :82-86
[10]   LOCATION OF THE DISULFIDE BONDS IN HUMAN PLASMA PREKALLIKREIN - THE PRESENCE OF 4 NOVEL APPLE DOMAINS IN THE AMINO-TERMINAL PORTION OF THE MOLECULE [J].
MCMULLEN, BA ;
FUJIKAWA, K ;
DAVIE, EW .
BIOCHEMISTRY, 1991, 30 (08) :2050-2056