Immunodetection of adenylyl cyclase protein in tissues

被引：13

作者：

Ishikawa, Y ^{[1
]}

Grant, BS

Okumura, S

Schwencke, C

Yanamoto, M

机构：

[1] Penn State Med Coll, Dept Med Cardiol, Dept Cellular & Mol Physiol, Weis Ctr Res, Danville, PA 17822 USA

[2] Yokohama City Univ, Sch Med, Dept Med Cardiol, Dept Physiol, Yokohama, Kanagawa 236004, Japan

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2000年 / 162卷 / 1-2期

关键词：

adenylyl cyclase isoforms; immunoblotting; protein; tissues; expression;

D O I：

10.1016/S0303-7207(00)00210-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Immunodetection of the adenylyl cyclase isoforms has been difficult in tissues because of its low quantity of protein expression. We have developed a one-step cellular protein purification method that enables a simple immunodetection of the adenylyl cyclase isoforms. The type I isoform was detected exclusively in the brain. The type III isoform was detected in the brain and lungs. Further, the protein expression of type III adenylyl cyclase in lungs changed ontogenically and was the lowest in neonates. Thus, the comparison of the amount of certain adenylyl cyclase isoforms protein in each tissue is now feasible using our method. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：107 / 112

页数：6

共 14 条

[1] IDENTIFICATION OF A SPECIALIZED ADENYLYL CYCLASE THAT MAY MEDIATE ODORANT DETECTION [J].