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Multilineage cells from adipose tissue as gene delivery vehicles

被引：104
作者：
Morizono, K
De Ugarte, DA
Zhu, M
Zuk, P
Elbarbary, A
Ashjian, P
Benhaim, P
Chen, ISY
Hedrick, MH
机构：
[1] Univ Calif Los Angeles, Sch Med, AIDS Inst, Dept Hematol Oncol, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Sch Med, Regenerat Bioengn & Repair Lab, Dept Surg, Los Angeles, CA 90024 USA
[3] Univ Calif Los Angeles, Sch Med, Regenerat Bioengn & Repair Lab, Dept Orthoped, Los Angeles, CA 90024 USA
来源：
HUMAN GENE THERAPY | 2003年 / 14卷 / 01期
关键词：
MESENCHYMAL STEM-CELLS; HIGH-EFFICIENCY TRANSDUCTION; MARROW STROMAL CELLS; HUMAN-FACTOR-IX; RETROVIRAL VECTOR; LENTIVIRUS VECTOR; LEUKEMIA-VIRUS; STABLE TRANSDUCTION; PROGENITOR CELLS; IN-VITRO;
D O I：
10.1089/10430340360464714
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have characterized a population of mesenchymal progenitor cells from adipose tissue, termed processed lipoaspirate (PLA) cells, which have multilineage potential similar to bone marrow-derived mesenchymal stem cells and are also easily expanded in culture. The primary benefit of using adipose tissue as a source of multilineage progenitor cells is its relative abundance and ease of procurement. We examined the infection of PLA cells with adenoviral, oncoretroviral, and lentiviral vectors. We demonstrate that PLA cells can be transduced with lentiviral vectors at high efficiency. PLA cells maintain transgene expression after differentiation into adipogenic and osteogenic lineages after lentiviral transduction. Therefore, PLA cells and lentiviral vectors may be an efficient combination for use as a therapeutic gene delivery vehicle.
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收藏
页码：59 / 66
页数：8
相关论文
共 50 条
[1]   High-efficiency transduction of human lymphoid progenitor cells and expression in differentiated T cells [J].
An, DS ;
Koyanagi, Y ;
Zhao, JQ ;
Akkina, R ;
Bristol, G ;
Yamamoto, N ;
Zack, JA ;
Chen, ISY .
JOURNAL OF VIROLOGY, 1997, 71 (02) :1397-1404
[2]  
[Anonymous], 1990, Bone Miner Res
[3]   Targeted adenoviral vectors [J].
Barnett, BG ;
Crews, CJ ;
Douglas, JT .
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2002, 1575 (1-3) :1-14
[4]   Baboon mesenchymal stem cells can be genetically modified to secrete human erythropoietin in vivo [J].
Bartholomew, A ;
Patil, S ;
Mackay, A ;
Nelson, M ;
Buyaner, D ;
Hardy, W ;
Mosca, J ;
Sturgeon, C ;
Siatskas, M ;
Mahmud, N ;
Ferrer, K ;
Deans, R ;
Moseley, A ;
Hoffman, R ;
Devine, SM .
HUMAN GENE THERAPY, 2001, 12 (12) :1527-1541
[5]   Mesenchymal stem cells: building blocks for molecular medicine in the 21st century [J].
Caplan, AI ;
Bruder, SP .
TRENDS IN MOLECULAR MEDICINE, 2001, 7 (06) :259-264
[6]  
CAPLAN AI, 2000, CLIN ORTHOP S, V37, pS67
[7]   Stable transduction of quiescent CD34+CD38- human hematopoietic cells by HIV-1-based lentiviral vectors [J].
Case, SS ;
Price, MA ;
Jordan, CT ;
Yu, XJ ;
Wang, LJ ;
Bauer, G ;
Haas, DL ;
Xu, DK ;
Stripecke, R ;
Naldini, L ;
Kohn, DB ;
Crooks, GM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (06) :2988-2993
[8]   LACK OF EXPRESSION FROM A RETROVIRAL VECTOR AFTER TRANSDUCTION OF MURINE HEMATOPOIETIC STEM-CELLS IS ASSOCIATED WITH METHYLATION IN-VIVO [J].
CHALLITA, PM ;
KOHN, DB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (07) :2567-2571
[9]   Retroviral vector-modified bone marrow stromal cells secrete biologically active factor IX in vitro and transiently deliver therapeutic levels of human factor IX to the plasma of dogs after reinfusion [J].
Cherington, V ;
Chiang, GG ;
McGrath, CA ;
Gaffney, A ;
Galanopoulos, T ;
Merrill, W ;
Bizinkauskas, CB ;
Hansen, M ;
Sobolewski, J ;
Levine, PH ;
Greenberger, JS ;
Hurwitz, DR .
HUMAN GENE THERAPY, 1998, 9 (10) :1397-1407
[10]   Retroviral expression in embryonic stem cells and hematopoietic stem cells [J].
Cherry, SR ;
Biniszkiewicz, D ;
van Parijs, L ;
Baltimore, D ;
Jaenisch, R .
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (20) :7419-7426
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