Energy and volume changes induced by photoinitiated proton releasing reaction with apomyoglobin

被引:8
作者
Choi, J [1 ]
Terazima, M [1 ]
机构
[1] Kyoto Univ, Dept Chem, Grad Sch Sci, Kyoto 6068502, Japan
关键词
D O I
10.1063/1.1515906
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
The photodissociation reaction of o-nitrobenzaldehyde (NBA) aqueous solution and of the NBA solution with a protein, apomyoglobin (ApoMb), were studied by the time-resolved transient grating (TG) technique. The amount of released thermal energy and the volume change accompanied with this reaction were determined by the TG and photoacoustic method. Without the protein, the photoproduct of NBA releases a proton in the solution (pH jump reaction). The time profile of the grating signal of NBA and ApoMb shows two diffusing species with diffusion coefficients of 1.39+/-0.15 and 0.14+/-0.02x10(-9) m(2) s(-1), respectively. From the diffusion coefficients, we suggest that the photoproduct of NBA induces a molecular recombination reaction between ApoMb and another small molecule or ion in the solution. (C) 2003 American Institute of Physics.
引用
收藏
页码:319 / 321
页数:3
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