2S methionine-rich protein (SSA) from sunflower seed is an IgE-binding protein

被引:31
作者
Kelly, JD [1 ]
Hefle, SL [1 ]
机构
[1] Univ Nebraska, Food Allergy Res & Resource Program, Lincoln, NE 68583 USA
关键词
amino-acid sequencing; IgE-binding protein; methionine-rich; SSA; sunflower seed;
D O I
10.1034/j.1398-9995.2000.00498.x
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Sunflower seed contains 2S albumins that in other crops have been associated with allergenicity. The sunflower seed methionine-rich 2S albumin (SSA) may be an IgE-binding protein responsible for anaphylactic reactions in some sunflower seed-sensitive subjects. The objective was to demonstrate that SSA is an IgE-binding protein. Methods: SSA was purified and the amino-acid sequence determined. The degree of purity of SSA was evaluated by silver staining, and its IgE-binding capacity by immunoblotting with serum from a subject with a convincing clinical history of anaphylaxis to sunflower seed. Results: The amino-acid sequence confirmed that the purified protein was the mature form of the methionine-rich storage protein SSA from sunflower seed (Helianthus annuus). The SSA was specifically recognized by IgE from the serum of the sunflower seed-allergic subject. Conclusions: SSA is an IgE-binding protein, and subjects allergic to sunflower seed whose IgE binds to SSA are at risk of developing allergic reactions if they consume SSA.
引用
收藏
页码:556 / 559
页数:4
相关论文
共 19 条
[1]   GENOTYPIC VARIATION AND POLYMORPHISM OF 2S ALBUMINS OF SUNFLOWER [J].
ANISIMOVA, IN ;
FIDO, RJ ;
TATHAM, AS ;
SHEWRY, PR .
EUPHYTICA, 1995, 83 (01) :15-23
[2]  
AXELSSON IGK, 1994, ALLERGY, V49, P514
[3]   SUNFLOWER SEED PROTEINS - CHARACTERIZATION AND SUBUNIT COMPOSITION OF THE GLOBULIN FRACTION [J].
DALGALARRONDO, M ;
RAYMOND, J ;
AZANZA, JL .
JOURNAL OF EXPERIMENTAL BOTANY, 1984, 35 (160) :1618-1628
[4]   CLONING AND EXPRESSION OF THE MAJOR ALLERGEN FROM YELLOW MUSTARD SEEDS, SIN-A-I [J].
DELAPENA, MAG ;
VILLALBA, M ;
GARCIALOPEZ, JL ;
RODRIGUEZ, R .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 190 (02) :648-653
[5]  
EGOROV TA, 1996, FEBS LETT, V396, P2
[6]  
Iwaya M, 1994, Arerugi, V43, P696
[7]  
Kelly JD, 1999, J ALLERGY CLIN IMMUN, V103, pS103
[8]   LOW-MOLECULAR-WEIGHT ALBUMINS FROM SUNFLOWER SEED - IDENTIFICATION OF A METHIONINE-RICH ALBUMIN [J].
KORTT, AA ;
CALDWELL, JB .
PHYTOCHEMISTRY, 1990, 29 (09) :2805-2810
[9]   AMINO-ACID AND CDNA SEQUENCES OF A METHIONINE-RICH 2S PROTEIN FROM SUNFLOWER SEED (HELIANTHUS-ANNUUS L) [J].
KORTT, AA ;
CALDWELL, JB ;
LILLEY, GG ;
HIGGINS, TJV .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 195 (02) :329-334
[10]  
Lusas E., 1985, NEW PROTEIN FOODS SE, P393