Immobilisation of anti-Listeria in a polypyrrole film

被引：30

作者：

Minett, AI ^{[1
]}

Barisci, JN ^{[1
]}

Wallace, GG ^{[1
]}

机构：

[1] Univ Wollongong, Intelligent Polymer Res Inst, Wollongong, NSW 2522, Australia

来源：

REACTIVE & FUNCTIONAL POLYMERS | 2002年 / 53卷 / 2-3期

基金：

澳大利亚研究理事会;

关键词：

Listeria; polypyrrole films; polymer; antibody; biosensors;

D O I：

10.1016/S1381-5148(02)00175-X

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The incorporation of antibody to Listeria into polypyrrole films during electropolymerisation and the covalent attachment of these antibodies to the conducting polymer backbone after polymerisation have been investigated. While the former approach provides a simple and convenient means of immobilisation, enzyme-linked immunosorbent assay tests have shown that covalent attachment produces a more bioactive surface and greatly improved reproducibility. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：217 / 227

页数：11

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