Infrared-based third and second harmonic generation imaging of cornea

被引：16

作者：

Chen, Szu-Yu ^{[1
]}

Yu, Han-Chieh ^{[3
]}

Wang, I-Jong ^{[3
]}

Sun, Chi-Kuang ^{[1
,2
]}

机构：

[1] Natl Taiwan Univ, Grad Inst Photon & Optoelect, Dept Elect Engn, Taipei 10617, Taiwan

[2] Acad Sinica, Res Ctr Appl Sci, Taipei 115, Taiwan

[3] Natl Taiwan Univ Hosp, Grad Inst Pathol, Dept Pathol, Taipei 100, Taiwan

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2009年 / 14卷 / 04期

关键词：

third harmonic generation; second harmonic generation; infrared based; cornea; mouse eye; high penetrability; high resolution; VIVO CONFOCAL MICROSCOPY; OPTICAL COHERENCE TOMOGRAPHY; HARMONIC-GENERATION; DEVELOPMENTAL BIOLOGY; REFRACTIVE SURGERY; LASER; LIGHT; TISSUES; MEDIA;

D O I：

10.1117/1.3183805

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

The cornea functions as an optical lens and plays an important role in vision. For corneal diagnosis and treatment such as refractive surgery, a microscopic imaging system with a 3-D cellular resolution and retinal safety is strongly desired. Recently, confocal and multiphoton microscopies have been applied to corneal imaging with visible to near-infrared light sources. To increase retinal safety, an infrared light source is be needed. In this work, an infrared-based third and second harmonic generation microscopic study of mouse eyes is reported with similar to 700-mu m penetrability and high cellular resolution. This study provides a critical reference for future development of infrared-based corneal imaging. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3183805]

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