Identification and light-induced expression of a novel gene of NADPH-protochlorophyllide oxidoreductase isoform in Arabidopsis thaliana

被引：112

作者：

Oosawa, N ^{[1
]}

Masuda, T ^{[1
]}

Awai, K ^{[1
]}

Fusada, N ^{[1
]}

Shimada, H ^{[1
]}

Ohta, H ^{[1
]}

Takamiya, K ^{[1
]}

机构：

[1] Tokyo Inst Technol, Fac Biosci & Biotechnol, Dept Biol Sci, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

FEBS LETTERS | 2000年 / 474卷 / 2-3期

关键词：

chlorophyll biosynthesis; chloroplast biogenesis; greening; NADPH-protochlorophyllide oxidoreductase; Arabidopsis thaliana;

D O I：

10.1016/S0014-5793(00)01568-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Arabidopsis thaliana, we identified a novel gene of a NADPH-protochlorophyllide oxidoreductase (POR) isoform, which catalyzes the light-dependent protochlorophyllide a reduction in the chlorophyll. (Chl) biosynthetic pathway, The deduced amino acid sequence of the novel FOR isoform (PORC) showed significant identities (similar to 75%) with the previously isolated tyro FOR isoforms of A. thaliana. Contrasting with these FOR isoforms, the PORC transcript increased in etiolated seedlings by illumination, and was dominantly expressed in immature and mature tissues. The present results demonstrated that Cbl biosynthesis and chloroplast biogenesis in A. thaliana are controlled by three FOR isoforms, which are differentially controlled by light and development. (C) 2000 Federation of European Biochemical Societies.

引用

页码：133 / 136

页数：4

共 13 条

[1] IDENTIFICATION OF NADPH-PROTOCHLOROPHYLLIDE OXIDOREDUCTASE-A AND OXIDOREDUCTASE-B - A BRANCHED PATHWAY FOR LIGHT-DEPENDENT CHLOROPHYLL BIOSYNTHESIS IN ARABIDOPSIS-THALIANA [J].