Asparagine synthetase activity in paediatric acute leukaemias:: AML-M5 subtype shows lowest activity

被引:41
作者
Dübbers, A
Würthwein, G
Müller, HJ
Schulze-Westhoff, P
Winkelhorst, M
Kurzknabe, E
Lanvers, C
Pieters, R
Kaspers, GJL
Creutzig, U
Ritter, J
Boos, J
机构
[1] Univ Munster, Dept Paediat Haematol Oncol, D-4400 Munster, Germany
[2] Univ Rotterdam, Sophia Childrens Hosp, Div Oncol Haematol, Rotterdam, Netherlands
[3] Free Univ Amsterdam, Dept Paediat Haematol Oncol, Amsterdam, Netherlands
关键词
asparagine synthetase; asparaginase; acute lymphoblastic leukaemia; acute myelogenous leukaemia; childhood leukaemia;
D O I
10.1046/j.1365-2141.2000.02015.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Lack of sufficient cellular activity of asparagine synthetase (AS) in blast cells compared with normal tissue is thought to be the basis of the antileukaemic effect of L-asparaginase in acute lymphoblastic leukaemia (ALL), Although L-asparaginase is routinely used in ALL, its role and Value in the treatment of acute myelogenous leukaemia (AML) is still being discussed, To evaluate the pharmacological basis for L-asparaginase treatment, we established pretreatment monitoring of the intracellular AS activity in blast cells of patients with AML and ALL. There was no general difference in AS activity between ALL and AML samples, Significantly lower AS activity however, was found in the B-lineage ALL subgroups as well as AML-M5.
引用
收藏
页码:427 / 429
页数:3
相关论文
共 12 条
[1]   Prognostic significance of early response to a single dose of asparaginase in childhood acute lymphoblastic leukemia [J].
Asselin, BL ;
Kreissman, S ;
Coppola, DJ ;
Bernal, SD ;
Leavitt, PR ;
Gelber, RD ;
Sallan, SE ;
Cohen, HJ .
JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY, 1999, 21 (01) :6-12
[2]  
Capizzi R. J. S. H., 1993, CANC MED, P796
[3]   L-ASPARAGINASE RESISTANCE IN HUMAN LEUKEMIA - AS PARAGINE SYNTHETASE [J].
HASKELL, CM ;
CANELLOS, GP .
BIOCHEMICAL PHARMACOLOGY, 1969, 18 (10) :2578-&
[4]   INHIBITION OF RAT-LIVER ASPARAGINE SYNTHETASE BY CIBACRON BLUE F3GA [J].
HONGO, S ;
SATO, T .
ENZYME, 1984, 31 (03) :181-186
[5]  
HONGO S, 1990, LEUKEMIA, V4, P708
[6]   Amino acid control of asparagine synthetase: Relation to asparaginase resistance in human leukemia cells [J].
Hutson, RG ;
Kitoh, T ;
Amador, DAM ;
Cosic, S ;
Schuster, SM ;
Kilberg, MS .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1997, 272 (05) :C1691-C1699
[7]   In vitro cellular drug resistance and prognosis in newly diagnosed childhood acute lymphoblastic leukemia [J].
Kaspers, GJL ;
Veerman, AJP ;
Pieters, R ;
VanZantwijk, CH ;
Smets, LA ;
VanWering, ER ;
DenBerg, AV .
BLOOD, 1997, 90 (07) :2723-2729
[8]  
KASPERS GJL, 1998, ACUTE LEUKEMIAS, V7, P491
[9]  
Kitoh T, 1998, BLOOD, V92, p400A
[10]  
Kitoh T., 1996, Blood, V88, p86A