A novel detection strategy for odorant molecules based on controlled bioengineering of rat olfactory receptor 17

被引：82

作者：

Hou, Yanxia ^{[1
]}

Jaffrezic-Renault, Nicole

Martelet, Claude

Zhang, Aidong

Minic-Vidic, Jasmina

Gorojankina, Tatiana

Persuy, Marie-Annick

Pajot-Augy, Edith

Salesse, Roland

Akimov, Vladimir

Reggiani, Lino

Pennetta, Cecilia

Alfinito, Eleonora

Ruiz, Oscar

Gomila, Gabriel

Samitier, Josep

Errachid, Abdelhamid

机构：

[1] Ecole Cent Lyon, Ctr Genie Elect Lyon, CEGELY, F-69134 Ecully, France

[2] Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China

[3] INRA, NOPA, RCC, F-78352 Jouy En Josas, France

[4] Univ Lecce, Dipartimento Ingn Innovaz, I-73100 Lecce, Italy

[5] CNR, INFM, Natl Nanotechnol Lab, I-73100 Lecce, Italy

[6] Lab Nanobioengn, E-08028 Barcelona, Spain

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 07期

关键词：

G protein-coupled receptors; olfactory receptor 17; self-assembled multilayer; electrochemical impedance spectroscopy; biosensor;

D O I：

10.1016/j.bios.2006.06.018

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this study, we report a dose-dependent detection of odorant molecules in solution by rat olfactory receptor 17 (OR 17) in its membrane fraction. The OR 17 is immobilized on a gold electrode by multilayer bioengineering based on a mixed self-assembled monolayer and biotin/avidin system, which allows for a well-controlled immobilization of the bioreceptor within its lipid environment. The odorant detection is electronically performed in a quantitative manner by electrochemical impedance spectroscopy (EIS) measurements on samples and controls. circle dot 2006 Elsevier B.V. All rights reserved.

引用

页码：1550 / 1555

页数：6

共 30 条

[1] Chronopotentiometry and Faradaic impedance spectroscopy as signal transduction methods for the biocatalytic precipitation of an insoluble product on electrode supports: routes for enzyme sensors, immunosensors and DNA sensors
Alfonta, L
Bardea, A
Khersonsky, O
Katz, E
Willner, I
[J]. BIOSENSORS & BIOELECTRONICS, 2001, 16 (9-12) : 675 - 687
[2] The molecular receptive range of an odorant receptor
Araneda, RC
Kini, AD
Firestein, S
[J]. NATURE NEUROSCIENCE, 2000, 3 (12) : 1248 - 1255
[3] Bardea A, 2000, ELECTROANAL, V12, P1097, DOI 10.1002/1521-4109(200010)12:14<1097::AID-ELAN1097>3.3.CO
[4] 2-O
[5] Micropatterned immobilization of a G protein-coupled receptor and direct detection of G protein activation
Bieri, C
Ernst, OP
Heyse, S
Hofmann, KP
Vogel, H
[J]. NATURE BIOTECHNOLOGY, 1999, 17 (11) : 1105 - 1108
[6] A NOVEL MULTIGENE FAMILY MAY ENCODE ODORANT RECEPTORS - A MOLECULAR-BASIS FOR ODOR RECOGNITION
BUCK, L
AXEL, R
[J]. CELL, 1991, 65 (01) : 175 - 187
[7] Crasto C, 2001, GENOME BIOL, V2
[8] Odorant receptors: a plethora of G-protein-coupled receptors
Dryer, L
Berghard, A
[J]. TRENDS IN PHARMACOLOGICAL SCIENCES, 1999, 20 (10) : 413 - 417
[9] Advances in the production, immobilization, and electrical characterization of olfactory receptors for olfactory nanobiosensor development
Gomila, G.
Casuso, I.
Errachid, A.
Ruiz, O.
Pajot, E.
Minic, J.
Gorojankina, T.
Persuy, M. A.
Aioun, J.
Salesse, R.
Bausells, J.
Villanueva, G.
Rius, G.
Hou, Y.
Jaffrezic, N.
Pennetta, C.
Alfinito, E.
Akimov, V.
Reggiani, L.
Ferrari, G.
Fumagalli, L.
Sampietro, M.
Samitier, J.
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2006, 116 (1-2) : 66 - 71
[10] Harper WJ, 2001, ADV EXP MED BIOL, V488, P59

← 1 2 3 →