Nitrosative stress inhibits the aminophospholipid translocase resulting in phosphatidylserine externalization and macrophage engulfment - Implications for the resolution of inflammation

被引:67
作者
Tyurina, Yulia Y.
Basova, Liana V.
Konduru, Nagarjun V.
Tyurin, Vladimir A.
Potapovich, Ala I.
Cai, Peter
Bayir, Hulya
Stoyanovsky, Detcho
Pitt, Bruce R.
Shvedova, Anna A.
Fadeel, Bengt
Kagan, Valerian E. [1 ]
机构
[1] Univ Pittsburgh, Ctr Free Rad & Antioxidant Hlth, Dept Environm & Occupat Hlth, Pittsburgh, PA 15219 USA
[2] Univ Pittsburgh, Dept Pharmacol, Pittsburgh, PA 15219 USA
[3] Univ Pittsburgh, Dept Crit Care Med, Pittsburgh, PA 15219 USA
[4] Univ Pittsburgh, Dept Surg, Pittsburgh, PA 15219 USA
[5] Univ Pittsburgh, Inst Canc, Pittsburgh, PA 15219 USA
[6] NIOSH, Morgantown, WV 26505 USA
[7] Karolinska Inst, Inst Environm Med, Div Biochem Toxicol, S-17177 Stockholm, Sweden
关键词
MEMBRANE PHOSPHOLIPID SCRAMBLASE; RED-BLOOD-CELLS; NITRIC-OXIDE; APOPTOTIC CELLS; S-NITROSYLATION; TRANSBILAYER MOVEMENT; PLASMA-MEMBRANE; PHAGOCYTE RECOGNITION; NEUTROPHIL APOPTOSIS; REACTIVE OXYGEN;
D O I
10.1074/jbc.M606950200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Macrophage recognition of apoptotic cells depends on externalization of phosphatidylserine (PS), which is normally maintained within the cytosolic leaflet of the plasma membrane by aminophospholipid translocase (APLT). APLT is sensitive to redox modifications of its -SH groups. Because activated macrophages produce reactive oxygen and nitrogen species, we hypothesized that macrophages can directly participate in apoptotic cell clearance by S-nitrosylation/oxidation and inhibition of APLT causing PS externalization. Here we report that exposure of target HL-60 cells to nitrosative stress inhibited APLT, induced PS externalization, and enhanced recognition and elimination of "nitrosatively" modified cells by RAW 264.7 macrophages. Using S-nitroso-L-cysteine-ethyl ester (SNCEE) and S-nitrosoglutathione (GSNO) that cause intracellular and extracellular trans-nitrosylation of proteins, respectively, we found that SNCEE (but not GSNO) caused significant S-nitrosylation/ oxidation of thiols in HL-60 cells. SNCEE also strongly inhibited APLT, activated scramblase, and caused PS externalization. However, SNCEE did not induce caspase activation or nuclear condensation/fragmentation suggesting that PS externalization was dissociated from the common apoptotic pathway. Dithiothreitol reversed SNCEE-induced S-nitrosylation, APLT inhibition, and PS externalization. SNCEE but not GSNO stimulated phagocytosis of HL-60 cells. Moreover, phagocytosis of target cells by lipopolysaccharide-stimulated macrophages was significantly suppressed by an NO center dot scavenger, DAF-2. Thus, macrophage-induced nitrosylation/oxidation plays an important role in cell clearance, and hence in the resolution of inflammation.
引用
收藏
页码:8498 / 8509
页数:12
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