Isolation and characterization of microsatellite DNA primers in burrowing owl (Athene cunicularia)

被引:12
作者
Korfanta, NM [1 ]
Schable, NA
Glenn, TC
机构
[1] Univ Wyoming, Sch Environm & Nat Resources, Laramie, WY 82071 USA
[2] Savannah River Ecol Lab, Aiken, SC USA
[3] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA
来源
MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 04期
关键词
aves; microsatellites; owls; PCR; primer; strigiformes;
D O I
10.1046/j.1471-8286.2002.00326.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We developed polymerase chain reaction (PCR) primers to amplify 22 microsatellite DNA loci in burrowing owls (Strigiformes: (.)Athene cunicularia). The seven most easily amplified and scored polymorphic loci are presented. These show 3-19 alleles per locus and expected heterozygosities of 0.067-0.712. Inheritance at all loci conformed to Mendelian expectations among six owl families, and six loci conform to Hardy-Weinberg expectations.
引用
收藏
页码:584 / 585
页数:2
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