Enzymatic synthesis of 2-ethylhexyl esters of fatty acids by immobilized lipase from Candida sp 99-125

被引：68

作者：

He, XL ^{[1
]}

Chen, BQ ^{[1
]}

Tan, TW ^{[1
]}

机构：

[1] Beijing Univ Chem Technol, Inst Chem Engn, Dept Biochem Engn, Beijing 100029, Peoples R China

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2002年 / 18卷 / 4-6期

基金：

中国国家自然科学基金;

关键词：

immobilized lipase; enzymatic esterification; Candida sp 99-125 lipase; 2-ethylhexyl palmitate;

D O I：

10.1016/S1381-1177(02)00114-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 2-ethylhexyl esters of fatty acids were synthesized by immobilized lipase from Candida sp. 99-125. The reuse stability of immobilized lipase was at least four batches. The conditions of enzymatic synthesis of 2-ethylhexyl palmitate were optimized. In the system of petroleum ether, 10% (w/w) immobilized lipase was used in the esterfication of 2-ethyl hexanol (7.8 mmol) and pahnitic acid (7.8 mmol) at 40 degreesC with silica gel as the water absorbent. The esterification degree was 91% under these conditions. The purity of 2-ethylhexyl palmitate was 98% after purification consisting washing by water and evaporation to remove the organic solvent. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：333 / 339

页数：7

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