Alendronate Enhances Osteogenic Differentiation of Bone Marrow Stromal Cells: A Preliminary Study

被引：87

作者：

Kim, Hyung Keun ^{[2
,3
]}

Kim, Ji Hyun ^{[2
,3
]}

Abbas, Azlina Amir ^{[2
]}

Yoon, Taek Rim ^{[1
,2
,3
]}

机构：

[1] Chonnam Natl Univ, Hwasun Hosp, Ctr Joint Dis, Jeonnam 519809, South Korea

[2] Chonnam Natl Univ, Hwasun Hosp, Dept Orthopaed, Jeonnam 519809, South Korea

[3] Chonnam Natl Univ, Cardiovasc Res Inst, Kwangju, South Korea

来源：

CLINICAL ORTHOPAEDICS AND RELATED RESEARCH | 2009年 / 467卷 / 12期

关键词：

IN-VITRO; OSTEOCLAST FORMATION; MEVALONATE PATHWAY; GENE-EXPRESSION; RAT BONE; BISPHOSPHONATES; PROLIFERATION; INHIBITION; RESORPTION; FRACTURE;

D O I：

10.1007/s11999-008-0409-y

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

Alendronate inhibits osteoclastic activity. However, some studies suggest alendronate also has effects on osteoblast activity. We hypothesized alendronate would enhance osteoblastic differentiation without causing cytotoxicity of the osteoblasts. We evaluated the effect of alendronate on the osteogenic differentiation of mouse mesenchymal stem cells. D1 cells (multipotent mouse mesenchymal stem cells) were cultured in osteogenic differentiation medium for 7 days and then treated with alendronate for 2 days before being subjected to various tests using MTT assays, Alizarin Red, enzyme-linked immunosorbent assay, energy-dispersive xray spectrophotometry, reverse transcriptase-polymerase chain reaction, confocal microscopy, and flow cytometric analysis. D1 cells differentiated into osteoblasts in the presence of osteogenic differentiation medium as confirmed by positive Alizarin Red S staining, increased alkaline phosphatase activity and osteocalcin mRNA expression, a calcium peak by energy-dispersive xray spectrophotometry, and by positive immunofluorescence staining against CD44. Osteogenic differentiation was enhanced after treatment with alendronate as confirmed by Alizarin Red S staining, elevated alkaline phosphatase activity and osteocalcin mRNA expression, a greater calcium peak by energy-dispersive xray spectrophotometry, and by immunofluorescence staining against CD44 by flow cytometric analysis. These data suggest alendronate enhances osteogenic differentiation when treated with mouse mesenchymal stem cells in osteogenic differentiation medium.

引用

页码：3121 / 3128

页数：8

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