Relative amounts of tissues in mature wheat (Triticum aestivum L.) grain and their carbohydrate and phenolic acid composition

被引:228
作者
Barron, C. [1 ]
Surget, A. [1 ]
Rouau, X. [1 ]
机构
[1] INRA, UMR IATE, UMII, CIRAD, F-34060 Montpellier 1, France
关键词
wheat grain; aleurone; germ; scutellum; pericarp; arabinoxylan; phenolic acids;
D O I
10.1016/j.jcs.2006.07.004
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Hand dissection of mature grains from two common wheats (Triticum aestivum L., cv. Caphorn and cv. Crousty) were performed to quantitatively assess their tissue composition and to obtain homogeneous samples of embryonic axis, scutellum, starchy endosperm, aleurone layer, hyaline layer, outer pericarp and a composite layer made up of testa + hyaline layer + inner pericarp. Polymeric neutral sugar and phenolic acid contents of the samples were determined and used to identify specific composition patterns in each tissue irrespective of the cultivar. The scutellum and embryonic axis showed the lowest amount of carbohydrates with similar relative amounts of arabinose and xylose (Ara + Xyl), but the scutellum differed from the embryonic axis in its high phenolic acid, in particular ferulate dehydrodimer, content. The peripheral layers of the grains were mainly composed of cell wall polysaccharides, chiefly arabinoxylans but with differing structures. The hyaline layer was mostly composed of arabinoxylan with extremely low Ara/Xyl ratio (0.1), with high amounts of ferulic acid monomers and hence very weakly crosslinked. The aleurone layer differed from the outer pericarp by its much lower Ara/Xyl ratio and lower amounts of ferulic acid dimers and trimers. High proportions of ether-linked phenolic acids (released by alkali at 170 degrees C were detected specifically in the seed coat and tissues in the crease region. The possible application of biochemical markers found in the various tissues to monitor wheat grain fractionation processes is discussed. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:88 / 96
页数:9
相关论文
共 35 条
[1]   Wheat bran tissue fractionation using biochemical markers [J].
Antoine, C ;
Peyron, S ;
Lullien-Pellerin, V ;
Abecassis, J ;
Rouau, X .
JOURNAL OF CEREAL SCIENCE, 2004, 39 (03) :387-393
[2]   Individual contribution of grain outer layers and their cell wall structure to the mechanical properties of wheat bran [J].
Antoine, C ;
Peyron, S ;
Mabille, F ;
Lapierre, C ;
Bouchet, B ;
Abecassis, J ;
Rouau, X .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (07) :2026-2033
[3]   A chemical and histological study on the effect of (1→4)-β-endo-xylanase treatment on wheat bran [J].
Benamrouche, S ;
Crônier, D ;
Debeire, P ;
Chabbert, B .
JOURNAL OF CEREAL SCIENCE, 2002, 36 (02) :253-260
[4]   A SIMPLE AND RAPID PREPARATION OF ALDITOL ACETATES FOR MONOSACCHARIDE ANALYSIS [J].
BLAKENEY, AB ;
HARRIS, PJ ;
HENRY, RJ ;
STONE, BA .
CARBOHYDRATE RESEARCH, 1983, 113 (02) :291-299
[5]  
BRADBURY D, 1956, CEREAL CHEM, V33, P373
[6]  
BRADBURY D, 1956, CEREAL CHEM, V33, P342
[7]  
BRADBURY D, 1956, CEREAL CHEM, V33, P361
[8]   Cell wall hydroxycinnamates in wild rice (Zizania aquatica L.) insoluble dietary fibre [J].
Bunzel, M ;
Allerdings, E ;
Sinwell, V ;
Ralph, J ;
Steinhart, H .
EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2002, 214 (06) :482-488
[9]   Isolation and identification of a ferulic acid dehydrotrimer from saponified maize bran insoluble fiber [J].
Bunzel, M ;
Ralph, J ;
Funk, C ;
Steinhart, H .
EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2003, 217 (02) :128-133
[10]   Isolation of homogeneous fractions from wheat water-soluble arabinoxylans. Influence of the structure on their macromolecular characteristics [J].
Dervilly, G ;
Saulnier, L ;
Roger, P ;
Thibault, JF .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (02) :270-278