Anti-tumor CD8+ T cell immunity elicited by HIV-1-based virus-like particles incorporating HPV-16 E7 protein

被引：41

作者：

Di Bonito, Paola ^{[2
]}

Grasso, Felicia ^{[2
]}

Mochi, Stefania ^{[2
]}

Petrone, Linda ^{[2
]}

Fanales-Belasio, Emanuele ^{[1
]}

Mei, Arianna ^{[2
]}

Cesolini, Armando ^{[2
]}

Laconi, Giuseppe ^{[2
]}

Conrad, Heinke ^{[3
]}

Bernhard, Helga ^{[3
]}

Dembek, Claudia J. ^{[4
]}

Cosma, Antonio ^{[4
,5
]}

Santini, Stefano M. ^{[6
]}

Lapenta, Caterina ^{[6
]}

Donati, Simona ^{[6
]}

Muratori, Claudia ^{[1
]}

Giorgi, Colomba ^{[2
]}

Federico, Maurizio ^{[1
]}

机构：

[1] Ist Super Sanita, Natl AIDS Ctr, I-00161 Rome, Italy

[2] Ist Super Sanita, Dept Infect Parasit & Immunomediated Dis, I-00161 Rome, Italy

[3] Tech Univ Munich, Klinikum Rechts Isar, Dept Hematol Oncol, D-8000 Munich, Germany

[4] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Virol, Neuherberg, Germany

[5] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Clin Cooperat Grp Immune Monitoring, Neuherberg, Germany

[6] Ist Super Sanita, Dept Cell Biol & Neurosci, I-00161 Rome, Italy

来源：

VIROLOGY | 2009年 / 395卷 / 01期

关键词：

Virus-like particles; HPV-E7; CTLs; HIV-1; Nef; Cross-presentation; Protein delivery; CLASS-I MOLECULES; THERAPEUTIC VACCINATION; DENDRITIC CELLS; PLASMID DNA; NEF; ANTIGEN; GENERATION; VACCINES; NEUTRALIZATION; IMMUNIZATION;

D O I：

10.1016/j.virol.2009.09.012

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Here we report a novel strategy for the induction of CD8(+) T cell adaptive immune response against viral and tumor antigens. This approach relies on high levels of incorporation in HIV-1 VLPs of a mutant of HIV-1 Nef (Nef(mut)) which can act as anchoring element for foreign proteins. By in vitro assay, we found that VLP-associated Nef(mut) is efficiently cross-presented by antigen presenting cells. Inoculation in mice of VLPs incorporating the HPV-16 E7 protein fused to Nef(mut) led to an anti-E7 CD8(+) T cell response much stronger than that elicited by E7 recombinant protein inoculated with incomplete Freund's adjuvant and correlating with well-detectable anti-E7 CTL activity. Most relevantly, mice immunized with Nef(mut)-E7 VLPs developed a protective immune response against tumors induced by E7 expressing tumor cells. These results make Nef(mut) VLPs a promising candidate for new vaccine strategies focused on the induction of CD8(+) T cell immunity. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：45 / 55

页数：11

共 53 条

[1]

Plasmid DNA and viral vector-based vaccines for the treatment of cancer [J].

Anderson, Richard J. ;

Schneider, Joerg .

VACCINE, 2007, 25 :B24-B34

[2]

IDENTIFICATION OF H-2K(B) BINDING AND IMMUNOGENIC PEPTIDES FROM HUMAN PAPILLOMA-VIRUS TUMOR-ANTIGENS E6 AND E7 [J].

BAUER, S ;

HEEG, K ;

WAGNER, H ;

LIPFORD, GB .

SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1995, 42 (03) :317-323

[3]

Advances in dendritic cell-based therapeutic vaccines for cervical cancer [J].

Bellone, Stefania ;

Pecorelli, Sewio ;

Cannon, Martin J. ;

Santin, Alessandro D. .

EXPERT REVIEW OF ANTICANCER THERAPY, 2007, 7 (10) :1473-1486

[4]

High-risk human papillomavirus E7 expression reduces cell-surface MHC class I molecules and increases susceptibility to natural killer cells [J].

Bottley, G. ;

Watherston, O. G. ;

Hiew, Y-L ;

Norrild, B. ;

Cook, G. P. ;

Blair, G. E. .

ONCOGENE, 2008, 27 (12) :1794-1799

[5]

BOURGAULT VI, 2004, CANCER RES, V64, P8761

[6]

Therapeutic vaccination for HPV induced cervical cancers [J].

Brinkman, Joeli A. ;

Hughes, Sarah H. ;

Stone, Pamela ;

Caffrey, Angela S. ;

Muderspach, Laila I. ;

Roman, Lynda D. ;

Weber, Jeffrey S. ;

Kast, W. Martin .

DISEASE MARKERS, 2007, 23 (04) :337-351

[7]

Nef association with human immunodeficiency virus type 1 virions and cleavage by the viral protease [J].

Bukovsky, AA ;

Dorfman, T ;

Weimann, A ;

Gottlinger, HG .

JOURNAL OF VIROLOGY, 1997, 71 (02) :1013-1018

[8]

Baculovirus-derived human immunodeficiency virus type 1 virus-like particles activate dendritic cells and induce ex vivo T-cell responses [J].

Buonaguro, L. ;

Tornesello, M. L. ;

Tagliamonte, M. ;

Gallo, R. C. ;

Wang, L. X. ;

Kamin-Lewis, R. ;

Abdelwahab, S. ;

Lewis, G. K. ;

Buonaguro, F. M. .

JOURNAL OF VIROLOGY, 2006, 80 (18) :9134-9143

[9]

MHC-I-restricted presentation of HIV-1 virion antigens without viral replication [J].

Buseyne, F ;

Le Gall, S ;

Boccaccio, C ;

Abastado, JP ;

Lifson, JD ;

Arthur, LO ;

Rivière, Y ;

Heard, JM ;

Schwartz, O .

NATURE MEDICINE, 2001, 7 (03) :344-349

[10]

Therapeutic vaccination with MVA-HIV-1 nef elicits Nef-specific T-helper cell responses in chronically HIV-1 infected individuals [J].

Cosma, A ;

Nagaraj, R ;

Bühler, S ;

Hinkula, J ;

Busch, DH ;

Sutter, G ;

Goebel, FD ;

Erfle, V .

VACCINE, 2003, 22 (01) :21-29

← 1 2 3 4 5 6 →