Tracking of human Y receptors in living cells -: A fluorescence approach

被引：25

作者：

Boehme, Ilka ^{[1
]}

Moerl, Karin ^{[1
]}

Bamming, Darja ^{[1
]}

Meyer, Cindy ^{[1
]}

Beck-Sickinger, Annette G. ^{[1
]}

机构：

[1] Univ Leipzig, Inst Biochem, Fac Biosci Pharmacol & Psychol, D-04103 Leipzig, Germany

来源：

PEPTIDES | 2007年 / 28卷 / 02期

关键词：

Y receptors; live cell imaging; GPCR trafficking; fluorescent tag; EYFP; Lumio (TM)-tag; SNAP (TM)-tag;

D O I：

10.1016/j.peptides.2006.08.042

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Non-invasive methods for studying biological processes in living cells have become very important, also in the field of GPCR biochemistry. Great advancements in the application of fluorescence techniques as well as in the development and improvement of novel fluorophores allow the visualization of dynamic processes. Using these technologies, problems concerning receptor biosynthesis, internalization, recycling and degradation can be investigated. Here we compare the application of the different fluorescent tags EYFP, Lumio (TM) and SNAP (TM) to track hY(1) and hY(5) receptors in living cells. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：226 / 234

页数：9

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