Separation and quantification of components of edible fat utilizing open tubular columns in SFC. Sample introduction by direct injection and SFE coupled on-line to SFC

Geometric isomers of fatty acids were separated by open tubular columns in SFC. The concentration of the analytes varied between 9 and 16 mu g mL(-1). Quantification of triglycerides with repeatability better than 20 % were obtained in a home made SFE - SFC unit. A four point calibration curve for both trimyristin and tripalmitin was developed with correlation coefficients of 0.998 and 0.9998, respectively. The limit of quantification was approximately 3 ng for both components. Supercritical CO2 as extraction solvent in matrix containing lipids increased the recovery of cholesterol by a factor of three. Using immobilized lipase in on-line SFE SFC quantification of cholesterol in spray dried egg yolk was possible.