Enhancement of lung-colonizing potential of murine tumor cell lines co-cultivated with activated macrophages

被引：15

作者：

Cecconi, O ^{[1
]}

Calorini, L ^{[1
]}

Mannini, A ^{[1
]}

Mugnai, G ^{[1
]}

Ruggieri, S ^{[1
]}

机构：

[1] UNIV FLORENCE,INST GEN PATHOL,I-50134 FLORENCE,ITALY

来源：

CLINICAL & EXPERIMENTAL METASTASIS | 1997年 / 15卷 / 02期

关键词：

C-parvum-elicited macrophages; thioglycollate-elicited macrophages; murine fibrosarcoma lines; B16-F10 melanoma cells; lung colonies;

D O I：

10.1023/A:1018440508189

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In order to explore the influence of activated macrophages on tumor cells, we cultured a series of weakly metastatic clones isolated from the murine T3 fibrosarcoma line (T3 clones) and the B16-F10 melanoma cells on feeder layers of C. parvum- or thioglycollate-elicited macrophages, or 'resident' (unstimulated) macrophages. Go-cultivation,vith C. parvum-elicited macrophages, but not with resident macrophages, induced an increase of the lung-colonizing potential of T3 clones and B16-F10 cells. An enhancement of lung-colonizing potential was also found in tumor cells grown in media conditioned by C. parvum-elicited macrophages. Thioglycollate-elicited macrophages also generated a pro-clonogenic activity which was however effective only on T3 clones but not on B16-F10 cells. The increase in the lung-colonizing potential of tumor cells stimulated by activated macrophages was retained to some degree after subcultivation in tissue culture medium or transplantation into syngeneic animals. In conclusion, our data support the notion that macrophages at different states of activation may enhance lung colonization of tumor cells by inducing a partially stable change of phenotype.

引用

页码：94 / 101

页数：8

共 76 条

[1] THE CELL BIOLOGY OF MACROPHAGE ACTIVATION [J].