Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells

被引：252

作者：

Gruss, OJ

Wittmann, M

Yokoyama, H

Pepperkok, R

Kufer, T

Silljé, H

Karsenti, E

Mattaj, IW

Vernos, I

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

[2] Max Planck Inst Biochem, Dept Cell Biol, D-82152 Martinsried, Germany

来源：

NATURE CELL BIOLOGY | 2002年 / 4卷 / 11期

关键词：

D O I：

10.1038/ncb870

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In Xenopus laevis egg extracts, TPX2 is required for the Ran-GTP-dependent assembly of microtubules around chromosomes. Here we show that interfering with the function of the human homologue of TPX2 in HeLa cells causes defects in microtubule organization during mitosis. Suppressing the expression of human TPX2 by RNA interference leads to the formation of two microtubule asters that do not interact and do not form a spindle. Our results suggest that in vivo, even in the presence of duplicated centrosomes, spindle formation requires the function of TPX2 to generate a stable bipolar spindle with overlapping antiparallel microtubule arrays. This indicates that chromosome-induced microtubule production is a general requirement for the formation of functional spindles in animal cells.

引用

页码：871 / 879

页数：9

共 39 条

[1] The GTPase Ran regulates chromosome positioning and nuclear envelope assembly in vivo [J].