Interest of immunomodulation as a mean to improve the preparation of polyclonal and monoclonal antibody reagents

被引:5
作者
Bouige, P [1 ]
Iscaki, S [1 ]
Budkowska, A [1 ]
Cosson, A [1 ]
Pillot, J [1 ]
机构
[1] INST PASTEUR,UNITE IMMUNOL MICROBIENNE,F-75724 PARIS 15,FRANCE
关键词
immunomodulation; monoclonal antibody; immunological reagent; anti-virus; anti-bacterium; SIgA;
D O I
10.1016/S0022-1759(96)00176-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Immunomodulation by monoclonal antibodies (mAbs) was investigated in mice in order to improve the preparation of antibody reagents. Three different types of representative immunogens were chosen: a human soluble protein (secretory immunoglobulin A, SIgA), a bacterial polysaccharide from E. coli K1 and an envelope protein from the hepatitis B virus. These Ag are all of importance for diagnosis and exhibit different levels of immunogenicity. Antibody-mediated enhancement was observed against restricted and defined regions of each immunogen i.e.: the Fab epitopes of SIgA, the preS1 domain of the HBV envelope and associated cell wall components of the capsular PS. The epitopes which were enhanced appeared to be different from those recognized by the modulating mAb. Negative modulations were also observed. Moreover, new epitopes seemed to be generated, In both cases the level and direction of the modulation were irrespective of isotypy and affinity of the mAbs. Interestingly the positive modulatory effect was found to be correlated with an in vitro assay based on the binding of immune complex to antigen-presenting cells.
引用
收藏
页码:27 / 37
页数:11
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