Effect-directed fractionation and identification of cytochrome P4501A-inducing halogenated aromatic hydrocarbons in a contaminated sediment

被引:54
作者
Brack, W
Schirmer, K
Kind, T
Schrader, S
Schüürmann, G
机构
[1] UFZ Helmholtz Ctr Environm Res, Dept Chem Ecotoxicol, D-04318 Leipzig, Germany
[2] UFZ Helmholtz Ctr Environm Res, Jr Res Grp Mol Anim Cell Toxicol, D-04318 Leipzig, Germany
[3] UFZ Helmholtz Ctr Environm Res, Dept Analyt Chem, D-04318 Leipzig, Germany
关键词
effect-directed fractionation; ethoxyresorufin-O-deethylase; toxicant identification; sediment extract;
D O I
10.1002/etc.5620211218
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
On the basis of a new fractionation method combined with in vitro ethoxyresorufin-O-deethylase (EROD) induction in a rainbow trout liver cell line (RTL-W1) and chemical analysis, halogenated aromatic hydrocarbons with dioxin-like activity were identified in a sediment extract from Bitterfeld, Germany. The fractionation method allowed a separation of different nonplanar and coplanar polychlorinated biphenyls (PCBs), polychlorinated naphthalenes (PCNs), dibenzo-p-dioxins (PCDDs), and dibenzofurans (PCDFs) with different degrees of chlorination. The dioxin-like activity at the investigated site could be quantitatively assigned to PCDD/Fs. Both PCBs and PCNs could be excluded as the cause of the measured effects on the basis of the fractionation procedure and bioanalytical results. Thus, the method allowed the chemical analysis to focus on PCDD/Fs, with significant reduction of the analytical expense. The EROD-induction potency of sediment-extract fractions was quantified, and toxicants were confirmed by the application of induction equivalent quantities on the basis of fixed-effect-level concentrations that exhibit 15% of the maximum induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin. This approach was designed to minimize methodological limitations due to superimposing inhibitory effects.
引用
收藏
页码:2654 / 2662
页数:9
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